Literature DB >> 2599352

Electroporation induced transformation of Bacteroides ruminicola and Bacteroides uniformis by plasmid DNA.

A M Thomson1, H J Flint.   

Abstract

Frequencies of greater than 10(5) transformants per microgram DNA were achieved in Bacteroides ruminicola F101 by electroporation of cells under anaerobic conditions, using the 19.5 kbp tetracycline resistance plasmid pRRI4. Similar procedures gave frequences of 10(6) erythromycin resistant transformants per microgram DNA with the shuttle plasmid pDP1 (19 kbp) in Bacteroides uniformis. Transformation of B. uniformis occurred at a far lower frequency (10(3) micrograms) when pDP1 DNA was derived from E. coli rather than B. uniformis.

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Year:  1989        PMID： 2599352     DOI： 10.1016/0378-1097(89)90178-x

Source DB:  PubMed          Journal:  FEMS Microbiol Lett        ISSN： 0378-1097            Impact factor:   2.742


  6 in total

1.  Sequence analysis and characterization of pOM1, a small cryptic plasmid from Butyrivibrio fibrisolvens, and its use in construction of a new family of cloning vectors for Butyrivibrios.

Authors:  M A Hefford; Y Kobayashi; S E Allard; R J Forster; R M Teather
Journal:  Appl Environ Microbiol       Date:  1997-05       Impact factor: 4.792

2.  Characterization of a Neocallimastix patriciarum cellulase cDNA (celA) homologous to Trichoderma reesei cellobiohydrolase II.

Authors:  S Denman; G P Xue; B Patel
Journal:  Appl Environ Microbiol       Date:  1996-06       Impact factor: 4.792

Review 3.  Gene transfer to plants by electroporation: methods and applications.

Authors:  Ibrahim Ilker Ozyigit
Journal:  Mol Biol Rep       Date:  2020-04-02       Impact factor: 2.316

4.  Conjugal transfer of a shuttle vector from the human colonic anaerobe Bacteroides uniformis to the ruminal anaerobe Prevotella (Bacteroides) ruminicola B(1)4.

Authors:  N B Shoemaker; K L Anderson; S L Smithson; G R Wang; A A Salyers
Journal:  Appl Environ Microbiol       Date:  1991-08       Impact factor: 4.792

5.  Partial characterization of a DNA restriction endonuclease from Ruminococcus flavefaciens FD-1 and its inhibition by site-specific adenine methylation.

Authors:  M Morrison; R I Mackie; B A White
Journal:  Appl Environ Microbiol       Date:  1992-01       Impact factor: 4.792

6.  A stable and efficient transformation system for Butyrivibrio fibrisolvens OB156.

Authors:  C E Beard; M A Hefford; R J Forster; S Sontakke; R M Teather; K Gregg
Journal:  Curr Microbiol       Date:  1995-02       Impact factor: 2.188
  6 in total

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