Literature DB >> 25992768

Lepr(db) mouse model of type 2 diabetes: pancreatic islet isolation and live-cell 2-photon imaging of intact islets.

Oanh H Do1, Jiun T Low1, Peter Thorn2.   

Abstract

Type 2 diabetes is a chronic disease affecting 382 million people in 2013, and is expected to rise to 592 million by 2035 (1). During the past 2 decades, the role of beta-cell dysfunction in type 2 diabetes has been clearly established (2). Research progress has required methods for the isolation of pancreatic islets. The protocol of the islet isolation presented here shares many common steps with protocols from other groups, with some modifications to improve the yield and quality of isolated islets from both the wild type and diabetic Lepr(db) (db/db) mice. A live-cell 2-photon imaging method is then presented that can be used to investigate the control of insulin secretion within islets.

Entities:  

Mesh：

Substances：

Year:  2015        PMID： 25992768      PMCID： PMC4542571          DOI： 10.3791/52632

Source DB:  PubMed          Journal:  J Vis Exp        ISSN： 1940-087X            Impact factor:   1.355


  11 in total

1.  Fusion pore dynamics and insulin granule exocytosis in the pancreatic islet.

Authors:  Noriko Takahashi; Takuya Kishimoto; Tomomi Nemoto; Takashi Kadowaki; Haruo Kasai
Journal:  Science       Date:  2002-08-23       Impact factor: 47.728

Review 2.  Five stages of evolving beta-cell dysfunction during progression to diabetes.

Authors:  Gordon C Weir; Susan Bonner-Weir
Journal:  Diabetes       Date:  2004-12       Impact factor: 9.461

3.  Global estimates of diabetes prevalence for 2013 and projections for 2035.

Authors:  L Guariguata; D R Whiting; I Hambleton; J Beagley; U Linnenkamp; J E Shaw
Journal:  Diabetes Res Clin Pract       Date:  2013-12-01       Impact factor: 5.602

4.  Chronic hyperglycemia, independent of plasma lipid levels, is sufficient for the loss of beta-cell differentiation and secretory function in the db/db mouse model of diabetes.

Authors:  Cecilie Kjørholt; Mia C Akerfeldt; Trevor J Biden; D Ross Laybutt
Journal:  Diabetes       Date:  2005-09       Impact factor: 9.461

5.  Diabetes, a new mutation in the mouse.

Authors:  K P Hummel; M M Dickie; D L Coleman
Journal:  Science       Date:  1966-09-02       Impact factor: 47.728

Review 6.  Diabetes mellitus and the β cell: the last ten years.

Authors:  Frances M Ashcroft; Patrik Rorsman
Journal:  Cell       Date:  2012-03-16       Impact factor: 41.582

7.  Glucose principally regulates insulin secretion in mouse islets by controlling the numbers of granule fusion events per cell.

Authors:  Jiun T Low; Justin M Mitchell; Oanh H Do; Jacinda Bax; Alicia Rawlings; Michael Zavortink; Garry Morgan; Robert G Parton; Herbert Y Gaisano; Peter Thorn
Journal:  Diabetologia       Date:  2013-09-01       Impact factor: 10.122

8.  Spontaneous type 2 diabetic rodent models.

Authors:  Yang-Wei Wang; Guang-Dong Sun; Jing Sun; Shu-Jun Liu; Ji Wang; Xiao-Hong Xu; Li-Ning Miao
Journal:  J Diabetes Res       Date:  2013-02-28       Impact factor: 4.011

9.  ScanImage: flexible software for operating laser scanning microscopes.

Authors:  Thomas A Pologruto; Bernardo L Sabatini; Karel Svoboda
Journal:  Biomed Eng Online       Date:  2003-05-17       Impact factor: 2.819

10.  The secretory deficit in islets from db/db mice is mainly due to a loss of responding beta cells.

Authors:  Oanh H Do; Jiun T Low; Herbert Y Gaisano; Peter Thorn
Journal:  Diabetologia       Date:  2014-04-06       Impact factor: 10.122
View more
  7 in total

1.  Confocal Imaging of Neuropeptide Y-pHluorin: A Technique to Visualize Insulin Granule Exocytosis in Intact Murine and Human Islets.

Authors:  Madina Makhmutova; Tao Liang; Herbert Gaisano; Alejandro Caicedo; Joana Almaça
Journal:  J Vis Exp       Date:  2017-09-13       Impact factor: 1.355

2.  ABCA12 regulates insulin secretion from β-cells.

Authors:  Gloria M Ursino; Ying Fu; Denny L Cottle; Nigora Mukhamedova; Lynelle K Jones; Hann Low; Ming Shen Tham; Wan Jun Gan; Natalie A Mellett; Partha P Das; Jacquelyn M Weir; Michael Ditiatkovski; Stacey Fynch; Peter Thorn; Helen E Thomas; Peter J Meikle; Helena C Parkington; Ian M Smyth; Dmitri Sviridov
Journal:  EMBO Rep       Date:  2020-02-18       Impact factor: 8.807

3.  The microRNA-124-iGluR2/3 pathway regulates glucagon release from alpha cells.

Authors:  Haiyang Zhang; Rui Liu; Ting Deng; Xia Wang; Hongmei Lang; Yanjun Qu; Jingjing Duan; Dingzhi Huang; Guoguang Ying; Yi Ba
Journal:  Oncotarget       Date:  2016-04-26

4.  Hyperuricemia Predisposes to the Onset of Diabetes via Promoting Pancreatic β-Cell Death in Uricase-Deficient Male Mice.

Authors:  Jie Lu; Yuwei He; Lingling Cui; Xiaoming Xing; Zhen Liu; Xinde Li; Hui Zhang; Hailong Li; Wenyan Sun; Aichang Ji; Yao Wang; Huiyong Yin; Changgui Li
Journal:  Diabetes       Date:  2020-04-20       Impact factor: 9.461

5.  Urate-lowering therapy alleviates atherosclerosis inflammatory response factors and neointimal lesions in a mouse model of induced carotid atherosclerosis.

Authors:  Jie Lu; Mingshu Sun; Xinjiang Wu; Xuan Yuan; Zhen Liu; Xiaojie Qu; Xiaopeng Ji; Tony R Merriman; Changgui Li
Journal:  FEBS J       Date:  2019-02-09       Impact factor: 5.542

Review 6.  A Practical Guide to Rodent Islet Isolation and Assessment Revisited.

Authors:  Kathryn L Corbin; Hannah L West; Samantha Brodsky; Nicholas B Whitticar; William J Koch; Craig S Nunemaker
Journal:  Biol Proced Online       Date:  2021-03-01       Impact factor: 3.244

7.  A Simple High Efficiency Protocol for Pancreatic Islet Isolation from Mice.

Authors:  Daniel Villarreal; Geetali Pradhan; Chia-Shan Wu; Clinton D Allred; Shaodong Guo; Yuxiang Sun
Journal:  J Vis Exp       Date:  2019-08-30       Impact factor: 1.355
  7 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.