Jiezhong Chen1, Xu-Feng Huang1. 1. 1 Illawarra Health and Medical Research Institute, University of Wollongong, Northfields Avenue, NSW 2522, Australia ; 2 School of Biomedical Sciences, University of Queensland, St Lucia, QLD 4072, Australia.
Abstract
BACKGROUND: Obesity has been found to be associated with colon cancer. However, the mechanism of this relationship is unclear and thus a good animal model is required. Our previous research showed that some mice developed diet-induced obesity (DIO) whilst others were diet-resistant (DR) when fed a high-fat diet. METHODS: In the present study, we have tested the effects of a high-fat diet on the formation of colon polyps induced by azoxymethane (AOM) in both DIO and DR mice. RESULTS: We found that the DIO mice have developed 2.5 times of polyps compared to the DR mice (P<0.05) and 3.4 times of polyps compared to the low fat fed mice (P<0.05). Although the DR mice tended to have more polyps than the low-fat diet fed mice, this was not statistically significant. The DIO mice could have an increased polyp formation due to obesity-related cancer risk factors and different gene expression from DR mice. CONCLUSIONS: DIO mice could be used as an appropriate model for studying obesity-associated colon cancer; however DR mice are not suitable because they don't show any significant weight gains to indicate obesity.
BACKGROUND:Obesity has been found to be associated with colon cancer. However, the mechanism of this relationship is unclear and thus a good animal model is required. Our previous research showed that some mice developed diet-induced obesity (DIO) whilst others were diet-resistant (DR) when fed a high-fat diet. METHODS: In the present study, we have tested the effects of a high-fat diet on the formation of colon polyps induced by azoxymethane (AOM) in both DIO and DR mice. RESULTS: We found that the DIO mice have developed 2.5 times of polyps compared to the DR mice (P<0.05) and 3.4 times of polyps compared to the low fat fed mice (P<0.05). Although the DR mice tended to have more polyps than the low-fat diet fed mice, this was not statistically significant. The DIO mice could have an increased polyp formation due to obesity-related cancer risk factors and different gene expression from DR mice. CONCLUSIONS: DIO mice could be used as an appropriate model for studying obesity-associated colon cancer; however DR mice are not suitable because they don't show any significant weight gains to indicate obesity.
Authors: Kandy T Velázquez; Reilly T Enos; Meredith S Carson; Taryn L Cranford; Jackie E Bader; Ioulia Chatzistamou; Udai P Singh; Prakash S Nagarkatti; Mitzi Nagarkatti; J Mark Davis; James A Carson; E Angela Murphy Journal: Am J Physiol Gastrointest Liver Physiol Date: 2016-09-08 Impact factor: 4.052
Authors: Erika L Garcia-Villatoro; Jennifer A A DeLuca; Evelyn S Callaway; Kimberly F Allred; Laurie A Davidson; Martha E Hensel; Rani Menon; Ivan Ivanov; Stephen H Safe; Arul Jayaraman; Robert S Chapkin; Clinton D Allred Journal: Am J Physiol Gastrointest Liver Physiol Date: 2020-01-06 Impact factor: 4.052