Mila M B P Salcedo1,2, Andrea P S Damin3, Grasiela Agnes3, Suzana A Pessini4, Patricia El Beitune4, Claudio O P Alexandre3, Kathleen M Schmeler5, Gustavo Py Gomes da Silveira4. 1. Department of Gynecology and Obstetrics of Federal University of Health Sciences (UFCSPA)/Santa Casa de Porto Alegre, Porto Alegre, RS, Brazil. mila@ufcspa.edu.br. 2. , Rua Prof. Annes Dias, 295, 1° Andar, CEP-90020-090, Porto Alegre, RS, Brazil. mila@ufcspa.edu.br. 3. Laboratory of Molecular Biology of UFCSPA, Porto Alegre, RS, Brazil. 4. Department of Gynecology and Obstetrics of Federal University of Health Sciences (UFCSPA)/Santa Casa de Porto Alegre, Porto Alegre, RS, Brazil. 5. Department of Gynecologic Oncology and Reproductive Medicine, The University of Texas M.D. Anderson Cancer Center, Houston, TX, USA.
Abstract
PURPOSE: To investigate the prevalence of human papillomavirus (HPV) in cervical samples of pregnant and non-pregnant women in South-Brazil. METHODS: A prospective study of 91 pregnant and 92 non-pregnant women with no previous history of cervical dysplasia or cancer was carried out. Cervical samples for HPV testing and cytology were collected in each trimester of pregnancy and in the puerperium for pregnant women and at matched intervals for the non-pregnant women. All samples were analyzed through PCR with consensus primers GP5+/GP6+. Genotyping was performed using specific primers. To control for confounding factors, the analysis of multivariate logistic regression was applied. The measure of odds ratio (OR) and the 95 % confidence interval (95 % CI) were used. The level of statistical significance was set at 5 % (P ≤ 0.05). RESULTS: HPV DNA was detected in 23/91 (25.3 %) cervical samples from the pregnant women and in 12/92 (13 %) cervical samples from non-pregnant women (P = 0.035). There was a significant association among cervical HPV infection and young age, number of lifetime sexual partners, and the presence of abnormal cervical cytology. HPV16 and HPV18 were the viral types more frequently detected. Out of the 23 HPV-positive pregnant women, 17 (73.9 %) had normal cervical cytology. CONCLUSION: Our results suggest a higher prevalence of HPV infection in pregnant vs. non-pregnant women. This finding may be related to the relative immunosuppression observed in pregnant women, outlining the importance of the appropriate monitoring of the viral infection in this specific population.
PURPOSE: To investigate the prevalence of human papillomavirus (HPV) in cervical samples of pregnant and non-pregnant women in South-Brazil. METHODS: A prospective study of 91 pregnant and 92 non-pregnant women with no previous history of cervical dysplasia or cancer was carried out. Cervical samples for HPV testing and cytology were collected in each trimester of pregnancy and in the puerperium for pregnant women and at matched intervals for the non-pregnant women. All samples were analyzed through PCR with consensus primers GP5+/GP6+. Genotyping was performed using specific primers. To control for confounding factors, the analysis of multivariate logistic regression was applied. The measure of odds ratio (OR) and the 95 % confidence interval (95 % CI) were used. The level of statistical significance was set at 5 % (P ≤ 0.05). RESULTS:HPV DNA was detected in 23/91 (25.3 %) cervical samples from the pregnant women and in 12/92 (13 %) cervical samples from non-pregnant women (P = 0.035). There was a significant association among cervical HPV infection and young age, number of lifetime sexual partners, and the presence of abnormal cervical cytology. HPV16 and HPV18 were the viral types more frequently detected. Out of the 23 HPV-positive pregnant women, 17 (73.9 %) had normal cervical cytology. CONCLUSION: Our results suggest a higher prevalence of HPV infection in pregnant vs. non-pregnant women. This finding may be related to the relative immunosuppression observed in pregnant women, outlining the importance of the appropriate monitoring of the viral infection in this specific population.
Entities:
Keywords:
HPV DNA; Human papillomavirus; Pregnancy
Authors: Elaine Regina Prudêncio da Silva; Albert Schiaveto de Souza; Taiana Gabriela Barbosa de Souza; Daniel Henrique Tsuha; Ana Rita Barbieri Journal: PLoS One Date: 2017-12-18 Impact factor: 3.240