Peter J Stahl1, Chava Cogan2, Akanksha Mehta3, Alex Bolyakov2, Darius A Paduch2, Marc Goldstein2. 1. Department of Urology, Columbia University College of Physicians and Surgeons, New York, New York. Electronic address: ps2192@columbia.edu. 2. Department of Urology, Weill Cornell Medical College, New York, New York. 3. Department of Urology, Emory University School of Medicine, Atlanta, Georgia.
Abstract
OBJECTIVE: To assess the concordance of sperm chromatin structure assay (SCSA) results, epifluorescence TUNEL assay results, and standard semen parameters. DESIGN: Prospective, observational study. SETTING: Tertiary referral andrology clinic. PATIENT(S): A total of 212 men evaluated for subfertility by a single physician. INTERVENTION(S): Clinical history, physical examination, semen analysis, SCSA, and TUNEL assay. MAIN OUTCOME MEASURE(S): Spearman's rank correlation coefficients (r) between SCSA DNA fragmentation index (DFI), percentage TUNEL-positive sperm, and semen analysis parameters. RESULT(S): There was a positive correlation between SCSA DFI and TUNEL (r = 0.31), but the strength of this correlation was weaker than has previously been reported. The discordance rate between SCSA and TUNEL in classifying patients as normal or abnormal was 86 of 212 (40.6%). The SCSA DFI was moderately negatively correlated with sperm concentration and motility. The TUNEL results were unrelated to standard semen parameters. CONCLUSION(S): The SCSA DFI and percentage TUNEL-positive sperm are moderately correlated measures of sperm DNA integrity but yield different results in a large percentage of patients. The DFI is well-correlated with semen analysis parameters, whereas TUNEL is not. These data indicate that the SCSA and TUNEL assay measure different aspects of sperm DNA integrity and should not be used interchangeably.
OBJECTIVE: To assess the concordance of sperm chromatin structure assay (SCSA) results, epifluorescence TUNEL assay results, and standard semen parameters. DESIGN: Prospective, observational study. SETTING: Tertiary referral andrology clinic. PATIENT(S): A total of 212 men evaluated for subfertility by a single physician. INTERVENTION(S): Clinical history, physical examination, semen analysis, SCSA, and TUNEL assay. MAIN OUTCOME MEASURE(S): Spearman's rank correlation coefficients (r) between SCSA DNA fragmentation index (DFI), percentage TUNEL-positive sperm, and semen analysis parameters. RESULT(S): There was a positive correlation between SCSA DFI and TUNEL (r = 0.31), but the strength of this correlation was weaker than has previously been reported. The discordance rate between SCSA and TUNEL in classifying patients as normal or abnormal was 86 of 212 (40.6%). The SCSA DFI was moderately negatively correlated with sperm concentration and motility. The TUNEL results were unrelated to standard semen parameters. CONCLUSION(S): The SCSA DFI and percentage TUNEL-positive sperm are moderately correlated measures of sperm DNA integrity but yield different results in a large percentage of patients. The DFI is well-correlated with semen analysis parameters, whereas TUNEL is not. These data indicate that the SCSA and TUNEL assay measure different aspects of sperm DNA integrity and should not be used interchangeably.
Authors: Raquel Ausejo; Juan Manuel Martínez; Noelia Mendoza; Alfonso Bolarin; M Teresa Tejedor; Maria Victoria Falceto Journal: Front Vet Sci Date: 2022-06-30
Authors: Sheryl T Homa; Anna M Vassiliou; Jesse Stone; Aideen P Killeen; Andrew Dawkins; Jingyi Xie; Farley Gould; Jonathan W A Ramsay Journal: Genes (Basel) Date: 2019-03-19 Impact factor: 4.096