Felice Rivellese1, Jolien Suurmond2, Kim Habets2, Annemarie L Dorjée2, Nandhini Ramamoorthi3, Michael J Townsend3, Amato de Paulis4, Gianni Marone4, Tom W J Huizinga2, Costantino Pitzalis5, René E M Toes2. 1. Leiden University Medical Center, Leiden, The Netherlands, University of Naples Federico II, Naples, Italy, and William Harvey Research Institute and Barts and The London School of Medicine and Dentistry, Queen Mary University of London, London, UK. 2. Leiden University Medical Center, Leiden, The Netherlands. 3. Genentech Research and Early Development, South San Francisco, California. 4. University of Naples Federico II, Naples, Italy. 5. William Harvey Research Institute and Barts and The London School of Medicine and Dentistry, Queen Mary University of London, London, UK.
Abstract
OBJECTIVE: Mast cells have been implicated in the pathogenesis of rheumatoid arthritis (RA). In particular, their activation by interleukin-33 (IL-33) has been linked to the development of arthritis in animal models. The aim of this study was to evaluate the functional responses of human mast cells to IL-33 in the context of RA. METHODS: Human mast cells were stimulated with IL-33 combined with plate-bound IgG or IgG anti-citrullinated protein antibodies (ACPAs), and their effects on monocyte activation were evaluated. Cellular interactions of mast cells in RA synovium were assessed by immunofluorescence analysis, and the expression of messenger RNA (mRNA) for mast cell-specific genes was evaluated in synovial biopsy tissue from patients with early RA who were naive to treatment with disease-modifying antirheumatic drugs. RESULTS: IL-33 induced the up-regulation of Fcγ receptor type IIa and enhanced the activation of mast cells by IgG, including IgG ACPAs, as indicated by the production of CXCL8/IL-8. Intriguingly, mast cell activation triggered with IL-33 and IgG led to the release of mediators such as histamine and IL-10, which inhibited monocyte activation. Synovial mast cells were found in contact with CD14+ monocyte/macrophages. Finally, mRNA levels of mast cell-specific genes were inversely associated with disease severity, and IL-33 mRNA levels showed an inverse correlation with the levels of proinflammatory markers. CONCLUSION: When human mast cells are activated by IL-33, an immunomodulatory phenotype develops, with human mast cells gaining the ability to suppress monocyte activation via the release of IL-10 and histamine. These findings, together with the presence of synovial mast cell-monocyte interactions and the inverse association between the expression of mast cell genes at the synovial level and disease activity, suggest that these newly described mast cell-mediated inhibitory pathways might have a functional relevance in the pathogenesis of RA.
OBJECTIVE: Mast cells have been implicated in the pathogenesis of rheumatoid arthritis (RA). In particular, their activation by interleukin-33 (IL-33) has been linked to the development of arthritis in animal models. The aim of this study was to evaluate the functional responses of human mast cells to IL-33 in the context of RA. METHODS:Human mast cells were stimulated with IL-33 combined with plate-bound IgG or IgG anti-citrullinated protein antibodies (ACPAs), and their effects on monocyte activation were evaluated. Cellular interactions of mast cells in RA synovium were assessed by immunofluorescence analysis, and the expression of messenger RNA (mRNA) for mast cell-specific genes was evaluated in synovial biopsy tissue from patients with early RA who were naive to treatment with disease-modifying antirheumatic drugs. RESULTS:IL-33 induced the up-regulation of Fcγ receptor type IIa and enhanced the activation of mast cells by IgG, including IgG ACPAs, as indicated by the production of CXCL8/IL-8. Intriguingly, mast cell activation triggered with IL-33 and IgG led to the release of mediators such as histamine and IL-10, which inhibited monocyte activation. Synovial mast cells were found in contact with CD14+ monocyte/macrophages. Finally, mRNA levels of mast cell-specific genes were inversely associated with disease severity, and IL-33 mRNA levels showed an inverse correlation with the levels of proinflammatory markers. CONCLUSION: When human mast cells are activated by IL-33, an immunomodulatory phenotype develops, with human mast cells gaining the ability to suppress monocyte activation via the release of IL-10 and histamine. These findings, together with the presence of synovial mast cell-monocyte interactions and the inverse association between the expression of mast cell genes at the synovial level and disease activity, suggest that these newly described mast cell-mediated inhibitory pathways might have a functional relevance in the pathogenesis of RA.
Authors: In Ah Choi; Sang Jin Lee; Won Park; Sung Hwan Park; Seung-Cheol Shim; Han Joo Baek; Dae-Hyun Yoo; Hyun Ah Kim; Soo Kon Lee; Yun Jong Lee; Young Eun Park; Hoon-Suk Cha; Eun Young Lee; Eun Bong Lee; Yeong Wook Song Journal: Arch Rheumatol Date: 2018-03-23 Impact factor: 1.472
Authors: Jérémie Sellam; Elodie Rivière; Alice Courties; Paul-Olivier Rouzaire; Barbara Tolusso; Edward M Vital; Paul Emery; Gianfranco Ferraccioli; Martin Soubrier; Bineta Ly; Houria Hendel Chavez; Yassine Taoufik; Maxime Dougados; Xavier Mariette Journal: Arthritis Res Ther Date: 2016-12-13 Impact factor: 5.156
Authors: S Karkampouna; M Kreulen; M C Obdeijn; P Kloen; A L Dorjée; F Rivellese; A Chojnowski; I Clark; Marianna Kruithof-de Julio Journal: Curr Mol Biol Rep Date: 2016-07-14