INTRODUCTION: The objectives of the present study were to determine the seroprevalence and identify the causative agent of brucellosis in small ruminants in Pakistan. METHODOLOGY: A total of 278 serum and 212 milk samples were collected from sheep and goats that had close contact with seropositive bovine herds. Data related to age, sex, location, and breed were collected on the sampling day. Serum and milk samples were initially screened using two different Rose Bengal plate test (RBPT) antigens and a milk ring test (MRT). Seropositive samples were subjected to bacterial isolation and PCR analysis using Brucella genus-specific (bcsp31) and Brucella species-specific (IS711 for Brucella abortus and Brucella melitensis) quantitative real-time polymerase chain reactions (qRT-PCR). RESULTS: Twenty-four (8.6%) serum samples were positive by RBPT. Twenty (9.4%) animals were positive for Brucella antibodies using MRT. No Brucella isolates were obtained from the examined blood and milk samples. Of the 24 seropositive serum samples, 18 (75%) were positive in the Brucella genus-specific (bcsp31) and Brucella abortus-specific (IS711) qRT-PCR, respectively. CONCLUSIONS: Brucella abortus was identified as causative agent of ovine and caprine brucellosis in Pakistan. Results of this study can be used for the development of an effective control and eradication strategy for brucellosis in livestock, especially small ruminants.
INTRODUCTION: The objectives of the present study were to determine the seroprevalence and identify the causative agent of brucellosis in small ruminants in Pakistan. METHODOLOGY: A total of 278 serum and 212 milk samples were collected from sheep and goats that had close contact with seropositive bovine herds. Data related to age, sex, location, and breed were collected on the sampling day. Serum and milk samples were initially screened using two different Rose Bengal plate test (RBPT) antigens and a milk ring test (MRT). Seropositive samples were subjected to bacterial isolation and PCR analysis using Brucella genus-specific (bcsp31) and Brucella species-specific (IS711 for Brucella abortus and Brucella melitensis) quantitative real-time polymerase chain reactions (qRT-PCR). RESULTS: Twenty-four (8.6%) serum samples were positive by RBPT. Twenty (9.4%) animals were positive for Brucella antibodies using MRT. No Brucella isolates were obtained from the examined blood and milk samples. Of the 24 seropositive serum samples, 18 (75%) were positive in the Brucella genus-specific (bcsp31) and Brucella abortus-specific (IS711) qRT-PCR, respectively. CONCLUSIONS:Brucella abortus was identified as causative agent of ovine and caprine brucellosis in Pakistan. Results of this study can be used for the development of an effective control and eradication strategy for brucellosis in livestock, especially small ruminants.
Authors: Yasmin ElTahir; Al Ghalya Al Toobi; Waleed Al-Marzooqi; Osman Mahgoub; Maryne Jay; Yannick Corde; Hadi Al Lawati; Shekar Bose; Abeer Al Hamrashdi; Kaadhia Al Kharousi; Nasseb Al-Saqri; Rudaina Al Busaidi; Eugene H Johnson Journal: Vet Med Sci Date: 2018-05-23
Authors: Aman Ullah Khan; Falk Melzer; Ashraf Hendam; Ashraf E Sayour; Iahtasham Khan; Mandy C Elschner; Muhammad Younus; Syed Ehtisham-Ul-Haque; Usman Waheed; Muhammad Farooq; Shahzad Ali; Heinrich Neubauer; Hosny El-Adawy Journal: Front Vet Sci Date: 2020-12-02
Authors: Arbab Saddique; Shahzad Ali; Shamim Akhter; Iahtasham Khan; Heinrich Neubauer; Falk Melzer; Aman Ullah Khan; Asima Azam; Hosny El-Adawy Journal: Int J Environ Res Public Health Date: 2019-10-23 Impact factor: 3.390
Authors: Usama Saeed; Shahzad Ali; Tooba Latif; Muhammad Rizwan; Anam Iftikhar; Syed Ghulam Mohayud Din Hashmi; Aman Ullah Khan; Iahtasham Khan; Falk Melzer; Hosny El-Adawy; Heinrich Neubauer Journal: Int J Environ Res Public Health Date: 2020-09-21 Impact factor: 3.390