M N Zarpellon1, A C Gales2, A L Sasaki3, G J Selhorst3, T C Menegucci3, C L Cardoso3, L B Garcia3, M C B Tognim4. 1. Departamento de Ciências Básicas da Saúde, Universidade Estadual de Maringá, PR, Brazil; Hospital Universitário de Maringá, Universidade Estadual de Maringá, PR, Brazil. 2. Universidade Federal de São Paulo, São Paulo, SP, Brazil. 3. Departamento de Ciências Básicas da Saúde, Universidade Estadual de Maringá, PR, Brazil. 4. Departamento de Ciências Básicas da Saúde, Universidade Estadual de Maringá, PR, Brazil. Electronic address: mcbtognim@uem.br.
Abstract
BACKGROUND: Contaminated surfaces play an important role in the transmission of certain pathogens that are responsible for healthcare-associated infections. Although previous studies have shown that meticillin-resistant Staphylococcus aureus (MRSA) can survive on dry surfaces at room temperature, no published data regarding vancomycin-intermediate S. aureus (VISA) are available to date. AIM: To compare the survival time on different types of surfaces, cell-surface hydrophobicity, adherence to abiotic surfaces and biofilm formation of meticillin-susceptible S. aureus (MSSA), MRSA and VISA. METHODS: Survival of the S. aureus strains was tested on latex, cotton fabric, vinyl flooring and formica. Cell-surface hydrophobicity was determined using the hydrocarbon interaction affinity method. Adhesion to abiotic surfaces was tested on granite, latex (gloves), glass, vinyl flooring and formica. Biofilm formation was evaluated at 6, 12, 24 and 48 h. FINDINGS: All of the samples survived on the vinyl flooring and formica for at least 40 days. VISA survived on both surfaces for more than 45 days. All of the strains were highly hydrophobic. VISA adhered to latex, vinyl flooring and formica. Biofilm formation increased for all of the tested strains within 6-24 h. CONCLUSION: VISA present high survival, adherence and cell-surface hydrophobicity. Therefore, as the treatment of patients with VISA is a significant challenge for clinicians, greater care with cleaning and disinfection of different types of surfaces in healthcare facilities is recommended because these may become important reservoirs of multi-resistant pathogens.
BACKGROUND: Contaminated surfaces play an important role in the transmission of certain pathogens that are responsible for healthcare-associated infections. Although previous studies have shown that meticillin-resistant Staphylococcus aureus (MRSA) can survive on dry surfaces at room temperature, no published data regarding vancomycin-intermediate S. aureus (VISA) are available to date. AIM: To compare the survival time on different types of surfaces, cell-surface hydrophobicity, adherence to abiotic surfaces and biofilm formation of meticillin-susceptible S. aureus (MSSA), MRSA and VISA. METHODS: Survival of the S. aureus strains was tested on latex, cotton fabric, vinyl flooring and formica. Cell-surface hydrophobicity was determined using the hydrocarbon interaction affinity method. Adhesion to abiotic surfaces was tested on granite, latex (gloves), glass, vinyl flooring and formica. Biofilm formation was evaluated at 6, 12, 24 and 48 h. FINDINGS: All of the samples survived on the vinyl flooring and formica for at least 40 days. VISA survived on both surfaces for more than 45 days. All of the strains were highly hydrophobic. VISA adhered to latex, vinyl flooring and formica. Biofilm formation increased for all of the tested strains within 6-24 h. CONCLUSION: VISA present high survival, adherence and cell-surface hydrophobicity. Therefore, as the treatment of patients with VISA is a significant challenge for clinicians, greater care with cleaning and disinfection of different types of surfaces in healthcare facilities is recommended because these may become important reservoirs of multi-resistant pathogens.
Authors: Marianne Gunell; Janne Haapanen; Kofi J Brobbey; Jarkko J Saarinen; Martti Toivakka; Jyrki M Mäkelä; Pentti Huovinen; Erkki Eerola Journal: Nanotechnol Sci Appl Date: 2017-11-14
Authors: Ziad W Jaradat; Qutaiba O Ababneh; Sherin T Sha'aban; Ayesha A Alkofahi; Duaa Assaleh; Anan Al Shara Journal: Pathog Glob Health Date: 2020-10-28 Impact factor: 2.894