Sueli Moreno Senna1,2, Marília Kalinne Torres1, Daíllo Augusto Pereira Lopes1, Maria Claudia Alheiros-Lira1, Diógenis Barbosa de Moura1, Valéria Rêgo Alves Pereira3, Francisco Carlos Amanajás de Aguiar2, José Candido Ferraz4, Carol Góis Leandro5,6. 1. Department of Nutrition, Federal University of Pernambuco, Recife, Brazil. 2. Department of Nursing, Academic Center of Vitoria de Santo Antao, Federal University of Pernambuco, Vitória de Santo Antão, Brazil. 3. Departament of Immunology, Institute of Research Aggeu Magalhães, FIOCRUZ/PE, Recife, Brazil. 4. Department of Physical Education and Sport Science, Academic Center of Vitoria de Santo Antao, Federal University of Pernambuco, Rua Alto do Reservatório, s/n, Bela Vista, Vitória de Santo Antão, PE, CEP 55608-680, Brazil. 5. Department of Nutrition, Federal University of Pernambuco, Recife, Brazil. carolleandro22@gmail.com. 6. Department of Physical Education and Sport Science, Academic Center of Vitoria de Santo Antao, Federal University of Pernambuco, Rua Alto do Reservatório, s/n, Bela Vista, Vitória de Santo Antão, PE, CEP 55608-680, Brazil. carolleandro22@gmail.com.
Abstract
PURPOSE: To evaluate the effects of a moderate physical training (T) on the blood and splenic lymphocytes subsets and the rate of apoptosis in adult offspring submitted to perinatal low-protein (LP) diet. METHODS: Male Wistar rats were divided according to their mother's diet: control (C, 17 % casein) and undernourished (LP, 8 % casein). At the 60th day, pups were submitted to moderate physical training (8 weeks, 5 days week(-1), 60 min day(-1), at 70 % of VO2max). After T period, pups received an injection of lipopolysaccharide (LPS). B, NK, and TCD3+ lymphocytes subsets were analyzed by flow cytometry. Spleen lymphocytes apoptosis was evaluated by DNA fragmentation, phosphatidylserine externalization (PSE), and mitochondrial transmembrane depolarization (MTD) using a flow cytometer. Plasma TNF-α concentrations were analyzed by ELISA. RESULTS: LP + LPS pups showed a higher percentage of blood B, CD4+, and NK and a reduction in TCD3+, CD8+ than C pups. The percentage of NK and CD3+ was restored in LP + T + LPS pups. In the spleen, T normalized the percentage of NK in LP + LPS pups. LP + LPS pups showed a higher percentage of cells with PSE and MTD than C + LPS pups that was attenuated by T. The concentration of TNF-α was higher in LP + LPS than C + LPS, but it was attenuated in LP + T + LPS pups. CONCLUSION: Moderate physical training was able to revert the effects of perinatal LP diet on circulation lymphocytes subsets and attenuated splenic lymphocytes apoptosis and plasma TNF-α concentrations.
PURPOSE: To evaluate the effects of a moderate physical training (T) on the blood and splenic lymphocytes subsets and the rate of apoptosis in adult offspring submitted to perinatal low-protein (LP) diet. METHODS: Male Wistar rats were divided according to their mother's diet: control (C, 17 % casein) and undernourished (LP, 8 % casein). At the 60th day, pups were submitted to moderate physical training (8 weeks, 5 days week(-1), 60 min day(-1), at 70 % of VO2max). After T period, pups received an injection of lipopolysaccharide (LPS). B, NK, and TCD3+ lymphocytes subsets were analyzed by flow cytometry. Spleen lymphocytes apoptosis was evaluated by DNA fragmentation, phosphatidylserine externalization (PSE), and mitochondrial transmembrane depolarization (MTD) using a flow cytometer. Plasma TNF-α concentrations were analyzed by ELISA. RESULTS: LP + LPS pups showed a higher percentage of blood B, CD4+, and NK and a reduction in TCD3+, CD8+ than C pups. The percentage of NK and CD3+ was restored in LP + T + LPS pups. In the spleen, T normalized the percentage of NK in LP + LPS pups. LP + LPS pups showed a higher percentage of cells with PSE and MTD than C + LPS pups that was attenuated by T. The concentration of TNF-α was higher in LP + LPS than C + LPS, but it was attenuated in LP + T + LPS pups. CONCLUSION: Moderate physical training was able to revert the effects of perinatal LP diet on circulation lymphocytes subsets and attenuated splenic lymphocytes apoptosis and plasma TNF-α concentrations.
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