Yumiko Uchida1, Yukihiro Morimoto2, Takao Uchiike3, Tomoyuki Kamamoto4, Tamaki Hayashi4, Ikuyo Arai4, Toshiya Nishikubo4, Yukihiro Takahashi4. 1. Division of Neonatal Intensive Care, Nara Medical University Hospital, Center of Perinatal Medicine, 840 Shijyo-cho, Kashihara, Nara 634-8522, Japan. Electronic address: rmbsh274@yahoo.co.jp. 2. Project Promotion Department, Technology and Engineering Division, Ushio Incorporated, 1194 Sazuchi, Bessyo-cho, Himeji, Hyogo 671-0224, Japan. 3. Central Clinical Laboratory, Nara Medical University Hospital, 840 Shijyo-cho, Kashihara, Nara 634-8522, Japan. 4. Division of Neonatal Intensive Care, Nara Medical University Hospital, Center of Perinatal Medicine, 840 Shijyo-cho, Kashihara, Nara 634-8522, Japan.
Abstract
OBJECTIVE: Phototherapy using blue light-emitting diodes (LED) is effective against neonatal jaundice. However, green light phototherapy also reduces unconjugated jaundice. We aimed to determine whether mixed blue and green light can relieve jaundice with minimal oxidative stress as effectively as either blue or green light alone in a rat model. METHODS: Gunn rats were exposed to phototherapy with blue (420-520 nm), filtered blue (FB; 440-520 nm without<440-nm wavelengths, FB50 (half the irradiance of filtered blue), mixed (filtered 50% blue and 50% green), and green (490-590 nm) LED irradiation for 24h. The effects of phototherapy are expressed as ratios of serum total (TB) and unbound (UB) bilirubin before and after exposure to each LED. Urinary 8-hydroxy-2'-deoxyguanosine (8-OHdG) was measured by HPLC before and after exposure to each LED to determine photo-oxidative stress. RESULTS: Values < 1.00 indicate effective phototherapy. The ratios of TB and UB were decreased to 0.85, 0.89, 1.07, 0.90, and 1.04, and 0.85, 0.94, 0.93, 0.89, and 1.09 after exposure to blue, filtered blue, FB50, and filtered blue mixed with green LED, respectively. In contrast, urinary 8-OHdG increased to 2.03, 1.25, 0.96, 1.36, 1.31, and 1.23 after exposure to blue, filtered blue, FB50, mixed, green LED, and control, indicating side-effects (> 1.00), respectively. CONCLUSIONS: Blue plus green phototherapy is as effective as blue phototherapy and it attenuates irradiation-induced oxidative stress. PRACTICE IMPLICATIONS: Combined blue and green spectra might be effective against neonatal hyperbilirubinemia.
OBJECTIVE: Phototherapy using blue light-emitting diodes (LED) is effective against neonatal jaundice. However, green light phototherapy also reduces unconjugated jaundice. We aimed to determine whether mixed blue and green light can relieve jaundice with minimal oxidative stress as effectively as either blue or green light alone in a rat model. METHODS:Gunn rats were exposed to phototherapy with blue (420-520 nm), filtered blue (FB; 440-520 nm without<440-nm wavelengths, FB50 (half the irradiance of filtered blue), mixed (filtered 50% blue and 50% green), and green (490-590 nm) LED irradiation for 24h. The effects of phototherapy are expressed as ratios of serum total (TB) and unbound (UB) bilirubin before and after exposure to each LED. Urinary 8-hydroxy-2'-deoxyguanosine (8-OHdG) was measured by HPLC before and after exposure to each LED to determine photo-oxidative stress. RESULTS: Values < 1.00 indicate effective phototherapy. The ratios of TB and UB were decreased to 0.85, 0.89, 1.07, 0.90, and 1.04, and 0.85, 0.94, 0.93, 0.89, and 1.09 after exposure to blue, filtered blue, FB50, and filtered blue mixed with green LED, respectively. In contrast, urinary 8-OHdG increased to 2.03, 1.25, 0.96, 1.36, 1.31, and 1.23 after exposure to blue, filtered blue, FB50, mixed, green LED, and control, indicating side-effects (> 1.00), respectively. CONCLUSIONS: Blue plus green phototherapy is as effective as blue phototherapy and it attenuates irradiation-induced oxidative stress. PRACTICE IMPLICATIONS: Combined blue and green spectra might be effective against neonatal hyperbilirubinemia.
Authors: M Taheri; M Darabyan; E Izadbakhsh; F Nouri; M Haghani; S A R Mortazavi; G Mortazavi; S M J Mortazavi; M Moradi Journal: J Biomed Phys Eng Date: 2017-06-01
Authors: S A R Mortazavi; S Parhoodeh; M A Hosseini; H Arabi; H Malakooti; S Nematollahi; G Mortazavi; L Darvish; S M J Mortazavi Journal: J Biomed Phys Eng Date: 2018-12-01