Hyun Jae Lee1, Jin Sung Park2, Yong Pill Yoon1, Ye Jin Shin3, Sang Kook Lee4, Yeong Shik Kim4, Jang-Hee Hong1, Kun Ho Son5, Choong Jae Lee6. 1. Department of Pharmacology, School of Medicine, Chungnam National University, 6 Munhwa-Dong, Joong-Gu, Daejeon 303-131, Republic of Korea. 2. Department of Orthopedic Surgery and Institute of Health Sciences, School of Medicine and Hospital, Gyeongsang National University, Jinju 660-702, Republic of Korea. 3. Department of Food Science and Nutrition, Andong National University, Andong 760-749, Republic of Korea. 4. Department of Pharmacy, College of Pharmacy, Seoul National University, Seoul 151-742, Republic of Korea. 5. Department of Orthopedic Surgery and Institute of Health Sciences, School of Medicine and Hospital, Gyeongsang National University, Jinju 660-702, Republic of Korea. Electronic address: sonkh@anu.ac.kr. 6. Department of Pharmacology, School of Medicine, Chungnam National University, 6 Munhwa-Dong, Joong-Gu, Daejeon 303-131, Republic of Korea. Electronic address: LCJ123@cnu.ac.kr.
Abstract
BACKGROUND: The root of Asparagus cochinchinensis (Lour.) Merr. has been utilized as mucoregulators and expectorants for controlling the airway inflammatory diseases in folk medicine. HYPOTHESIS/ PURPOSE: We investigated whether dioscin and methylprotodioscin isolated from the root of Asparagus cochinchinensis (Lour.) Merr. suppress the gene expression and production of airway MUC5AC mucin induced by phorbol ester and growth factor. STUDY DESIGN: Confluent NCI-H292 cells were pretreated with dioscin or methylprotodioscin for 30 min and then stimulated with EGF or PMA for 24 h. The MUC5AC mucin gene expression was measured by RT-PCR. Production of MUC5AC mucin protein was measured by ELISA. RESULTS: (1) Dioscin and methylprotodioscin suppressed the expression of MUC5AC mucin gene induced by EGF or PMA; (2) dioscin suppressed the production of MUC5AC mucin induced by either EGF at 10(-5) M (p < 0.05) and 10(-6) M (p < 0.05) or PMA at 10(-4) M (p < 0.05), 10(-5) M (p < 0.05) and 10(-6) M (p < 0.05); (3) methylprotodioscin also suppressed the production of MUC5AC mucin induced by either EGF at 10(-4) M (p < 0.05) or PMA at 10(-4) M (p < 0.05). CONCLUSION: These results suggest that dioscin and methylprotodioscin isolated from the root of Asparagus cochinchinensis suppress the gene expression and production of MUC5AC mucin, by directly acting on airway epithelial cells, and the results are consistent with the traditional use of Asparagus cochinchinensis as remedy for diverse inflammatory pulmonary diseases.
BACKGROUND: The root of Asparagus cochinchinensis (Lour.) Merr. has been utilized as mucoregulators and expectorants for controlling the airway inflammatory diseases in folk medicine. HYPOTHESIS/ PURPOSE: We investigated whether dioscin and methylprotodioscin isolated from the root of Asparagus cochinchinensis (Lour.) Merr. suppress the gene expression and production of airway MUC5AC mucin induced by phorbol ester and growth factor. STUDY DESIGN: Confluent NCI-H292 cells were pretreated with dioscin or methylprotodioscin for 30 min and then stimulated with EGF or PMA for 24 h. The MUC5AC mucin gene expression was measured by RT-PCR. Production of MUC5AC mucin protein was measured by ELISA. RESULTS: (1) Dioscin and methylprotodioscin suppressed the expression of MUC5AC mucin gene induced by EGF or PMA; (2) dioscin suppressed the production of MUC5AC mucin induced by either EGF at 10(-5) M (p < 0.05) and 10(-6) M (p < 0.05) or PMA at 10(-4) M (p < 0.05), 10(-5) M (p < 0.05) and 10(-6) M (p < 0.05); (3) methylprotodioscin also suppressed the production of MUC5AC mucin induced by either EGF at 10(-4) M (p < 0.05) or PMA at 10(-4) M (p < 0.05). CONCLUSION: These results suggest that dioscin and methylprotodioscin isolated from the root of Asparagus cochinchinensis suppress the gene expression and production of MUC5AC mucin, by directly acting on airway epithelial cells, and the results are consistent with the traditional use of Asparagus cochinchinensis as remedy for diverse inflammatory pulmonary diseases.
Authors: Yulin Ren; Bethany G Elkington; Joshua M Henkin; Kongmany Sydara; A Douglas Kinghorn; Djaja D Soejarto Journal: J Med Plant Res Date: 2021-12-31
Authors: Ju Hee Lee; Hun Jai Lim; Chan Woo Lee; Kun-Ho Son; Jong-Keun Son; Sang Kook Lee; Hyun Pyo Kim Journal: Evid Based Complement Alternat Med Date: 2015-08-25 Impact factor: 2.629