Literature DB >> 25977944

Photo-triggered fluorescent labelling of recombinant proteins in live cells.

Deokho Jung1, Kohei Sato, Kyoungmi Min, Akira Shigenaga, Juyeon Jung, Akira Otaka, Youngeun Kwon.   

Abstract

A method to photo-chemically trigger fluorescent labelling of proteins in live cells is developed. The approach is based on photo-caged split-intein mediated conditional protein trans-splicing reaction and enabled background-free fluorescent labelling of target proteins with the necessary spatiotemporal control.

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Year:  2015        PMID： 25977944     DOI： 10.1039/c5cc01067e

Source DB:  PubMed          Journal:  Chem Commun (Camb)        ISSN： 1359-7345            Impact factor:   6.222


  10 in total

1.  Efficient and wash-free labeling of membrane proteins using engineered Npu DnaE split-inteins.

Authors:  Euiyeon Lee; Kyoungmi Min; Young-Tae Chang; Youngeun Kwon
Journal:  Protein Sci       Date:  2018-09-24       Impact factor: 6.725

Review 2.  Chemoenzymatic Semisynthesis of Proteins.

Authors:  Robert E Thompson; Tom W Muir
Journal:  Chem Rev       Date:  2019-11-27       Impact factor: 60.622

3.  Proximity Induced Splicing Utilizing Caged Split Inteins.

Authors:  Josef A Gramespacher; Antony J Burton; Luis F Guerra; Tom W Muir
Journal:  J Am Chem Soc       Date:  2019-08-21       Impact factor: 15.419

Review 4.  Intein-Mediated Protein Engineering for Cell-Based Biosensors.

Authors:  Chungwon Kang; Keshab Lal Shrestha; San Kwon; Seungil Park; Jinsik Kim; Youngeun Kwon
Journal:  Biosensors (Basel)       Date:  2022-04-28

Review 5.  Modular protein domains: an engineering approach toward functional biomaterials.

Authors:  Charng-Yu Lin; Julie C Liu
Journal:  Curr Opin Biotechnol       Date:  2016-03-09       Impact factor: 9.740

6.  An Engineered Split Intein for Photoactivated Protein Trans-Splicing.

Authors:  Stanley Wong; Abdullah A Mosabbir; Kevin Truong
Journal:  PLoS One       Date:  2015-08-28       Impact factor: 3.240

7.  'Traceless' tracing of proteins - high-affinity trans-splicing directed by a minimal interaction pair.

Authors:  M Braner; A Kollmannsperger; R Wieneke; R Tampé
Journal:  Chem Sci       Date:  2015-12-21       Impact factor: 9.825

Review 8.  Strategies for Site-Specific Labeling of Receptor Proteins on the Surfaces of Living Cells by Using Genetically Encoded Peptide Tags.

Authors:  Philipp Wolf; Georgina Gavins; Annette G Beck-Sickinger; Oliver Seitz
Journal:  Chembiochem       Date:  2021-02-26       Impact factor: 3.164

9.  Intein Zymogens: Conditional Assembly and Splicing of Split Inteins via Targeted Proteolysis.

Authors:  Josef A Gramespacher; Adam J Stevens; Duy P Nguyen; Jason W Chin; Tom W Muir
Journal:  J Am Chem Soc       Date:  2017-06-07       Impact factor: 15.419

10.  In Cellulo Protein Semi-Synthesis from Endogenous and Exogenous Fragments Using the Ultra-Fast Split Gp41-1 Intein.

Authors:  Maniraj Bhagawati; Simon Hoffmann; Katharina S Höffgen; Jacob Piehler; Karin B Busch; Henning D Mootz
Journal:  Angew Chem Int Ed Engl       Date:  2020-09-11       Impact factor: 15.336
  10 in total

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