Artur Javmen1, Aušra Nemeikaitė-Čėnienė2, Maksim Bratchikov3, Saulius Grigiškis4, Fortūnatas Grigas5, Irena Jonauskienė2, Danguolė Zabulytė2, Mykolas Mauricas2. 1. Department of Immunology, State Scientific Research Institute Centre for Innovative Medicine, Vilnius, Lithuania JSC Biocentras, Vilnius, Lithuania arturas.biocentras@gmail.com. 2. Department of Immunology, State Scientific Research Institute Centre for Innovative Medicine, Vilnius, Lithuania. 3. Department of Immunology, State Scientific Research Institute Centre for Innovative Medicine, Vilnius, Lithuania Department of Physiology, Biochemistry, Microbiology and Laboratory Medicine, Faculty of Medicine, Vilnius University, Vilnius, Lithuania. 4. JSC Biocentras, Vilnius, Lithuania Department of Chemistry and Bioengineering, Vilnius Gediminas Technical University, Vilnius, Lithuania. 5. JSC Biocentras, Vilnius, Lithuania.
Abstract
AIM: β-Glucan is one of the most abundant polymers in nature and has been established as an immunomodulator. This compound has notable physiological effects on mammalian immune systems, including anti-tumor and anti-infective activities and can activate the immune response. It is considered that the immune-stimulating activities of β-glucan can depend on physicochemical parameters, such as molecular size. Saccharomyces cerevisiae, also known as baker's yeast, is a frequently used source of β-glucan. The aim of the experiments was to investigate how different Saccharomyces cerevisiae β-glucan preparations with different molecular size affect interferon-gamma (IFN-γ) production in BALB/c mice. MATERIALS AND METHODS: In vivo and in vitro BALB/c mouse models were used for the investigations. Different β-glucan preparations were orally administrated in the in vivo experiments. IFN-γ production in BALB/c mice was analyzed by enzyme-linked immunosorbent assay and measuring interferon-γ RNA concentration. RESULTS: The results showed that orally-administered β-glucan from S. cerevisiae enhanced IFN-γ production in BALB/c mice in the in vivo model, but not by mouse leukocytes in vitro. Moreover, water-soluble β-glucan enhanced IFN-γ production more effectively than did particulate β-glucan. CONCLUSION: IFN-γ plays an important role in immunity against viral and bacterial infections. Our experiments have shown that β-glucan preparations enhance IFN-γ production in BALB/c mice and can be potentially used for immune system stimulation in mammals. Current results may be used to develop soluble β-glucan nutritional supplements.
AIM: β-Glucan is one of the most abundant polymers in nature and has been established as an immunomodulator. This compound has notable physiological effects on mammalian immune systems, including anti-tumor and anti-infective activities and can activate the immune response. It is considered that the immune-stimulating activities of β-glucan can depend on physicochemical parameters, such as molecular size. Saccharomyces cerevisiae, also known as baker's yeast, is a frequently used source of β-glucan. The aim of the experiments was to investigate how different Saccharomyces cerevisiae β-glucan preparations with different molecular size affect interferon-gamma (IFN-γ) production in BALB/c mice. MATERIALS AND METHODS: In vivo and in vitro BALB/c mouse models were used for the investigations. Different β-glucan preparations were orally administrated in the in vivo experiments. IFN-γ production in BALB/c mice was analyzed by enzyme-linked immunosorbent assay and measuring interferon-γ RNA concentration. RESULTS: The results showed that orally-administered β-glucan from S. cerevisiae enhanced IFN-γ production in BALB/c mice in the in vivo model, but not by mouse leukocytes in vitro. Moreover, water-soluble β-glucan enhanced IFN-γ production more effectively than did particulate β-glucan. CONCLUSION: IFN-γ plays an important role in immunity against viral and bacterial infections. Our experiments have shown that β-glucan preparations enhance IFN-γ production in BALB/c mice and can be potentially used for immune system stimulation in mammals. Current results may be used to develop soluble β-glucan nutritional supplements.
Authors: Barry W Neun; Edward Cedrone; Timothy M Potter; Rachael M Crist; Marina A Dobrovolskaia Journal: Molecules Date: 2020-07-24 Impact factor: 4.411
Authors: Enas Mahmoud Amer; Saber H Saber; Ahmad Abo Markeb; Amal A Elkhawaga; Islam M A Mekhemer; Abdel-Naser A Zohri; Turki S Abujamel; Steve Harakeh; Elham A Abd-Allah Journal: Molecules Date: 2021-04-07 Impact factor: 4.411