Daniel Benito1, Paula Gómez1, Carmen Aspiroz2, Myriam Zarazaga1, Carmen Lozano1, Carmen Torres3. 1. Área Bioquímica y Biología Molecular, Universidad de La Rioja, 26006 Logroño, Spain. 2. Unidad de Microbiología. Hospital Royo Villanova, Zaragoza, Spain. 3. Área Bioquímica y Biología Molecular, Universidad de La Rioja, 26006 Logroño, Spain. Electronic address: carmen.torres@unirioja.es.
Abstract
OBJECTIVES: To conduct a study of Staphylococcus aureus carriage in members of a livestock-farmer's family with different degrees of animal contact, and to characterize the recovered isolates. METHODS: Nasal samples from 11 members of the family were taken in three sampling periods (every six months) (n=31), and 9 skin samples from superficial lesions were also obtained in 5 of them. Samples were analyzed for S. aureus susceptible (MSSA) and resistant to methicillin (MRSA). S. aureus isolates were tested for antibiotic-resistance phenotype and genotype and for the detection of virulence and IEC-system genes. Molecular typing of isolates was also performed (spa- and multilocus-sequence typing). RESULTS: Eighteen S. aureus isolates were recovered (1 MRSA and 17 MSSA) in the 40 samples analyzed. S. aureus was detected in nasal and skin samples of 7/11 and 4/5 of tested humans, respectively. The MRSA strain was detected in the skin lesion of a farmer with high animal contact, and carried the mecC gene, and was typed as ST130-CC130-t843. The 17 MSSA isolates were ascribed to 9 different spa-types and sequence types included in the clonal complexes CC22, CC30, CC45, CC121, and in the livestock-associated lineages CC9 and CC133. Six strains harbored eta or tsst-1 genes. Three of 18 strains lacked the immune-evasion-cluster (IEC) genes (MRSA-ST130, MSSA-ST1333, and MSSA-ST133), and the remaining isolates were ascribed to IEC type-A or -B. CONCLUSIONS: Animal-associated S. aureus lineages were detected in samples of the farmer's family, highlighting the detection of MSSA-CC133 and mecC-MRSA-ST130.
OBJECTIVES: To conduct a study of Staphylococcus aureus carriage in members of a livestock-farmer's family with different degrees of animal contact, and to characterize the recovered isolates. METHODS: Nasal samples from 11 members of the family were taken in three sampling periods (every six months) (n=31), and 9 skin samples from superficial lesions were also obtained in 5 of them. Samples were analyzed for S. aureus susceptible (MSSA) and resistant to methicillin (MRSA). S. aureus isolates were tested for antibiotic-resistance phenotype and genotype and for the detection of virulence and IEC-system genes. Molecular typing of isolates was also performed (spa- and multilocus-sequence typing). RESULTS: Eighteen S. aureus isolates were recovered (1 MRSA and 17 MSSA) in the 40 samples analyzed. S. aureus was detected in nasal and skin samples of 7/11 and 4/5 of tested humans, respectively. The MRSA strain was detected in the skin lesion of a farmer with high animal contact, and carried the mecC gene, and was typed as ST130-CC130-t843. The 17 MSSA isolates were ascribed to 9 different spa-types and sequence types included in the clonal complexes CC22, CC30, CC45, CC121, and in the livestock-associated lineages CC9 and CC133. Six strains harbored eta or tsst-1 genes. Three of 18 strains lacked the immune-evasion-cluster (IEC) genes (MRSA-ST130, MSSA-ST1333, and MSSA-ST133), and the remaining isolates were ascribed to IEC type-A or -B. CONCLUSIONS: Animal-associated S. aureus lineages were detected in samples of the farmer's family, highlighting the detection of MSSA-CC133 and mecC-MRSA-ST130.
Authors: Lucia Mališová; Vladislav Jakubů; Martin Musílek; Jana Kekláková; Helena Žemličková Journal: Microb Drug Resist Date: 2020-02-24 Impact factor: 3.431
Authors: Elena Moreno-Grúa; Sara Pérez-Fuentes; Asunción Muñoz-Silvestre; David Viana; Ana B Fernández-Ros; Celia Sanz-Tejero; Juan M Corpa; Laura Selva Journal: Front Microbiol Date: 2018-08-14 Impact factor: 5.640
Authors: Paula Gómez; Laura Ruiz-Ripa; Rosa Fernández-Fernández; Haythem Gharsa; Karim Ben Slama; Ursula Höfle; Myriam Zarazaga; Mark A Holmes; Carmen Torres Journal: Front Microbiol Date: 2021-03-25 Impact factor: 5.640