BACKGROUND: Previously, we reported that microRNA-181b (miR-181b) activates hepatic stellate cells partly through the phosphatase and tensin homolog deleted on chromosome 10 (PTEN)/Akt pathway. AIMS: The main objective of this study was to ascertain whether serum miR-181b expression is correlated with that of liver hepatitis B virus (HBV) DNA and disease progression in chronic hepatitis B (CHB) patients. METHODS: Serum miR-181b and liver HBV DNA levels were quantified in 64 CHB patients with real-time PCR. Liver fibrosis and necroinflammation were graded according to the Ishak scoring system. RESULTS: Serum miR-181b levels were evaluated in the CHB group, compared with healthy controls. Expression in patients with HBsAg (+) was higher than that in patients with HBsAg (-). Notably, serum miR-181b and liver HBV DNA levels were significantly correlated (P < 0.05). Serum miR-181 levels were higher in patients with serum HBV DNA > 10(3) IU/ml (P = 0.017), histologic activity index (HAI) >8 (P = 0.001) and fibrosis score >4 (P < 0.0001). Liver HBV DNA levels were higher in patients with abnormal alanine aminotransferase (ALT) values (P = 0.004), serum HBV DNA levels > 10(3) IU/ml (P = 0.034) and fibrosis score >4 (P = 0.006). Using multivariate logistic regression analysis, serum miR-181b was identified as an independent predictor of disease progression (OR 4.172, 95 % CI 1.838-9.473, P = 0.009 for HAI >8; OR 5.387, 95 % CI 2.067-14.036, P = 0.001 for fibrosis score >4). CONCLUSIONS: Serum miR-181b is correlated with liver and serum HBV DNA levels as well as disease progression in CHB.
BACKGROUND: Previously, we reported that microRNA-181b (miR-181b) activates hepatic stellate cells partly through the phosphatase and tensin homolog deleted on chromosome 10 (PTEN)/Akt pathway. AIMS: The main objective of this study was to ascertain whether serum miR-181b expression is correlated with that of liver hepatitis B virus (HBV) DNA and disease progression in chronic hepatitis B (CHB) patients. METHODS: Serum miR-181b and liver HBV DNA levels were quantified in 64 CHB patients with real-time PCR. Liver fibrosis and necroinflammation were graded according to the Ishak scoring system. RESULTS: Serum miR-181b levels were evaluated in the CHB group, compared with healthy controls. Expression in patients with HBsAg (+) was higher than that in patients with HBsAg (-). Notably, serum miR-181b and liver HBV DNA levels were significantly correlated (P < 0.05). Serum miR-181 levels were higher in patients with serum HBV DNA > 10(3) IU/ml (P = 0.017), histologic activity index (HAI) >8 (P = 0.001) and fibrosis score >4 (P < 0.0001). Liver HBV DNA levels were higher in patients with abnormal alanine aminotransferase (ALT) values (P = 0.004), serum HBV DNA levels > 10(3) IU/ml (P = 0.034) and fibrosis score >4 (P = 0.006). Using multivariate logistic regression analysis, serum miR-181b was identified as an independent predictor of disease progression (OR 4.172, 95 % CI 1.838-9.473, P = 0.009 for HAI >8; OR 5.387, 95 % CI 2.067-14.036, P = 0.001 for fibrosis score >4). CONCLUSIONS: Serum miR-181b is correlated with liver and serum HBV DNA levels as well as disease progression in CHB.
Authors: K Ishak; A Baptista; L Bianchi; F Callea; J De Groote; F Gudat; H Denk; V Desmet; G Korb; R N MacSween Journal: J Hepatol Date: 1995-06 Impact factor: 25.083
Authors: Chwan Hong Foo; Christina L Rootes; Karla Cowley; Glenn A Marsh; Cathryn M Gould; Celine Deffrasnes; Christopher J Cowled; Reuben Klein; Sarah J Riddell; Deborah Middleton; Kaylene J Simpson; Lin-Fa Wang; Andrew G D Bean; Cameron R Stewart Journal: PLoS Pathog Date: 2016-10-26 Impact factor: 6.823
Authors: Ana Garcia Garcia de Paredes; Nicolo Manicardi; Luis Tellez; Luis Ibañez; Felix Royo; Javier Bermejo; Carolina Blanco; Constantino Fondevila; Val Fernandez Lanza; Laura Garcia-Bermejo; Juan Manuel Falcon-Perez; Rafael Bañares; Jordi Gracia-Sancho; Agustin Albillos Journal: Hepatol Commun Date: 2020-12-02
Authors: Ana Garcia Garcia de Paredes; Càndid Villanueva; Carolina Blanco; Joan Genescà; Nicolo Manicardi; Juan Carlos Garcia-Pagan; Jose Luis Calleja; Carlos Aracil; Rosa M Morillas; Maria Poca; Beatriz Peñas; Salvador Augustin; Juan G Abraldes; Eldimar Alvarado; Félix Royo; Maria Laura Garcia-Bermejo; Juan Manuel Falcon-Perez; Rafael Bañares; Jaime Bosch; Jordi Gracia-Sancho; Agustin Albillos Journal: JHEP Rep Date: 2021-09-27