Soundharrajan Ilavenil1, Da Hye Kim2, Young-Il Jeong3, Mariadhas Valan Arasu4, Mayakrishnan Vijayakumar1, Ponnuraj Nagendra Prabhu5, Srisesharam Srigopalram6, Ki Choon Choi7. 1. Grassland and Forage division, National Institute of Animal Science, RDA, Republic of Korea-330-801. 2. The United Graduate School of Agricultural Sciences, Tottori University, Japan 680-8553. 3. School of Medicine, Pusan National University, Yangsan, Gyeongnam 626-770, Republic of Korea. 4. Department of Botany and Microbiology, Addiriyah Chair for Environmental Studies, College of Science, King Saud University, Riyadh, 11451, Saudi Arabia. 5. Department of Animal Sciences, University of Illinois, USA 61801. 6. Department of Horticulture, Sunchon National University, South Korea. 7. Grassland and Forage division, National Institute of Animal Science, RDA, Republic of Korea-330-801. Electronic address: choiwh@korea.kr.
Abstract
OBJECTIVE: To elucidate the key parameters associated with hydrogen peroxide induced oxidative stress and investigates the mechanism of trigonelline (TG) for reducing the H2O2 induced toxicity in H9c2 cells. METHODS: Cytotoxicity and antioxidant activity of TG was assessed by EZ-CYTOX kit. RNA extraction and cDNA synthesized according to the kit manufacture protocol. Apoptosis was measured by the Flowcytometry, general PCR and qPCR. RESULTS: It was found that the TG significantly rescued the morphology of the H9c2 cells. Treatment of cells with TG attenuated H2O2 induced cell deaths and improved the antioxidant activity. In addition, TG regulated the apoptotic gene caspase-3, caspase-9 and anti-apoptotic gene Bcl-2, Bcl-XL during H2O2 induced oxidative stress in H9c2 cells. These results were comparable with quercetin treatment. For evident, flow cytometer results also confirmed the TG significantly reduced the H2O2 induced necrosis and apoptosis in H9c2 cells. However, further increment of TG concentration against H2O2 could induce the necrosis and apoptosis along with H2O2. CONCLUSIONS: It is suggested that less than 125 μ M of TG could protect the cells from H2O2 induced cell damage by down regulating the caspases and up regulating the Bcl-2 and Bcl-XL expression. Therefore, we suggest the trigonelline could be useful for treatment of oxidative stress mediated cardiovascular diseases in future.
OBJECTIVE: To elucidate the key parameters associated with hydrogen peroxide induced oxidative stress and investigates the mechanism of trigonelline (TG) for reducing the H2O2 induced toxicity in H9c2 cells. METHODS:Cytotoxicity and antioxidant activity of TG was assessed by EZ-CYTOX kit. RNA extraction and cDNA synthesized according to the kit manufacture protocol. Apoptosis was measured by the Flowcytometry, general PCR and qPCR. RESULTS: It was found that the TG significantly rescued the morphology of the H9c2 cells. Treatment of cells with TG attenuated H2O2 induced cell deaths and improved the antioxidant activity. In addition, TG regulated the apoptotic gene caspase-3, caspase-9 and anti-apoptotic gene Bcl-2, Bcl-XL during H2O2 induced oxidative stress in H9c2 cells. These results were comparable with quercetin treatment. For evident, flow cytometer results also confirmed the TG significantly reduced the H2O2 induced necrosis and apoptosis in H9c2 cells. However, further increment of TG concentration against H2O2 could induce the necrosis and apoptosis along with H2O2. CONCLUSIONS: It is suggested that less than 125 μ M of TG could protect the cells from H2O2 induced cell damage by down regulating the caspases and up regulating the Bcl-2 and Bcl-XL expression. Therefore, we suggest the trigonelline could be useful for treatment of oxidative stress mediated cardiovascular diseases in future.
Authors: Alan Gonçalves Amaral; Isabela Aparecida Moretto; Flávia da Silva Zandonadi; Hans Rolando Zamora-Obando; Isabela Rocha; Alessandra Sussulini; André Alexandre de Thomaz; Regina Vincenzi Oliveira; Aline Mara Dos Santos; Ana Valéria Colnaghi Simionato Journal: Front Chem Date: 2022-04-08 Impact factor: 5.545
Authors: Monica Força Lima; Alan Gonçalves Amaral; Isabela Aparecida Moretto; Franckson Jhonne Torres Neves Paiva-Silva; Flávia Oliveira Borges Pereira; Coral Barbas; Aline Mara Dos Santos; Ana Valéria Colnaghi Simionato; Francisco Javier Rupérez Journal: Front Mol Biosci Date: 2022-06-29