| Literature DB >> 25974866 |
Allison M Murawski1, Johnathan L Nieves1, Maitreyi Chattopadhyay2, Megan Y Young2, Christine Szarko2, Holleh F Tajalli1, Tareq Azad1, Nina B Jean-Jacques1, Anne E Simon2, David B Kushner3.
Abstract
The 356 nt noncoding satellite RNA C (satC) of Turnip crinkle virus (TCV) is composed of 5' sequences from a second TCV satRNA (satD) and 3' sequences derived from TCV. SHAPE structure mapping revealed that 76 nt in the poorly-characterized satD-derived region form an extended hairpin (H2). Pools of satC in which H2 was replaced with 76, 38, or 19 random nt were co-inoculated with TCV helper virus onto plants and satC fitness assessed using in vivo functional selection (SELEX). The most functional progeny satCs, including one as fit as wild-type, contained a 38-39 nt H2 region that adopted a hairpin structure and exhibited an increased ratio of dimeric to monomeric molecules. Some progeny of satC with H2 deleted featured a duplication of 38 nt, partially rebuilding the deletion. Therefore, H2 can be replaced by a 38-39 nt hairpin, sufficient for overall structural stability of the 5' end of satC.Entities:
Keywords: SELEX; Satellite RNA; Turnip crinkle virus; Viral RNA evolution
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Year: 2015 PMID： 25974866 PMCID： PMC6016832 DOI： 10.1016/j.virol.2015.04.002
Source DB: PubMed Journal: Virology ISSN： 0042-6822 Impact factor: 3.616