| Literature DB >> 2597355 |
T Mantovaara1, H Pertoft, J Porath.
Abstract
Serum amyloid P component (SAP) has been purified from human serum by means of immobilized metal ion affinity chromatography (IMAC). It was selectively concentrated on carboxymethylated aspartic acid agarose (CM-Asp-agarose) loaded with calcium and, employing very mild conditions, purified to electrophoretical and immunological homogeneity in a single step amounting to about 1900-fold purification. As a purification method our procedure thus compares well with bio-specific affinity chromatography.Entities:
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Year: 1989 PMID： 2597355
Source DB: PubMed Journal: Biotechnol Appl Biochem ISSN： 0885-4513 Impact factor: 2.431