Yan Hao1, Xue-Bi Tian2, Tao-Tao Liu2, Cheng Liu2, Hong-Bing Xiang2, Jian-Guo Zhang3. 1. Department of Pediatrics, Tongji Hospital of Tongji Medical College, Huazhong University of Science and Technology Wuhan 430030, Hubei, People's Republic of China. 2. Department of Anesthesiology and Pain Medicine, Tongji Hospital of Tongji Medical College, Huazhong University of Science and Technology Wuhan 430030, Hubei, People's Republic of China. 3. Beijing Key Laboratory of Neurostimulation Beijing 100050, China ; Department of Functional Neurosurgery, Beijing Neurosurgical Institute, Capital Medical University Beijing, China ; Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University Beijing 100050, People's Republic of China.
Abstract
BACKGROUND AND OBJECTIVE: Separate studies have implicated the pedunculopontine tegmental nucleus (PPTg) in processing aversive stimuli to dopamine systems, and melanocortin-4 receptor (MC4R) are broadly expressed by the neurons in the PPTg, but the exact neurosubstrate underlying the regulation of dopamine systems by the central melanocortin pathway is poorly understood. METHODS: In this study, the PPTg of 6 adult mice expressing green fluorescent protein (GFP) under the control of the MC4R promoter was detected by fluorescence immunohistochemistry. RESULTS: A large number of GFP-positive neurons in the dissipated parts of PPTg (dpPPTg) were found, and approximately 50% of MC4R-GFP- positive neurons in the dpPPTg coexpressed tyrosine hydroxylase, a marker of dopamine neurons, indicating that they were dopaminergic. CONCLUSIONS: Our findings support the hypothesis that MC4R signaling in the dpPPTg may involve in the modulation of midbrain dopamine systems.
BACKGROUND AND OBJECTIVE: Separate studies have implicated the pedunculopontine tegmental nucleus (PPTg) in processing aversive stimuli to dopamine systems, and melanocortin-4 receptor (MC4R) are broadly expressed by the neurons in the PPTg, but the exact neurosubstrate underlying the regulation of dopamine systems by the central melanocortin pathway is poorly understood. METHODS: In this study, the PPTg of 6 adult mice expressing green fluorescent protein (GFP) under the control of the MC4R promoter was detected by fluorescence immunohistochemistry. RESULTS: A large number of GFP-positive neurons in the dissipated parts of PPTg (dpPPTg) were found, and approximately 50% of MC4R-GFP- positive neurons in the dpPPTg coexpressed tyrosine hydroxylase, a marker of dopamine neurons, indicating that they were dopaminergic. CONCLUSIONS: Our findings support the hypothesis that MC4R signaling in the dpPPTg may involve in the modulation of midbrain dopamine systems.
Entities:
Keywords:
Melanocortin-4 receptor; dopamine; the pedunculopontine tegmental nucleus
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