Hongxia Li1, Xiangyu Meng2, Yue Gao3, Shaohua Cai2. 1. Department of Respiratory Medicine, South Building P. R. China. 2. Department of Respiratory Medicine, Special Inpatient Unit, Chinese PLA General Hospital Beijing 100853, P. R. China. 3. Institute of Radiation Medicine, Academy of Military Medical Sciences Beijing 100039, P. R. China.
Abstract
OBJECTIVE: To investigate the alterations of microparticles in acute respiratory distress syndrome (ARDS) in rats. METHODS: 18 Wistar male rats were randomly divided into three groups: no intervention, sham (saline control) group and ARDS group (LPS induced). Blood was collected from abdominal aorta and microparticles were extracted through multiple rounds of centrifugation. Particles were analyzed by flow cytometry and transmission electron microscope. RESULTS: The circulating concentration of total microparticles of rats with ARDS induced by lipopolysaccharide (LPS) did not change compared with other two groups. However, ARDS rats expressed higher concentration of leukocyte- and endothelium- derived microparticles in the three groups. CONCLUSION: Our results indicate that leukocyte and endothelial cell-derived particles may play an important role in ARDS. Thus it is important not only to monitor total microparticle levels but also the phenotypes, which may contribute to the prevention and early treatment of ARDS.
OBJECTIVE: To investigate the alterations of microparticles in acute respiratory distress syndrome (ARDS) in rats. METHODS: 18 Wistar male rats were randomly divided into three groups: no intervention, sham (saline control) group and ARDS group (LPS induced). Blood was collected from abdominal aorta and microparticles were extracted through multiple rounds of centrifugation. Particles were analyzed by flow cytometry and transmission electron microscope. RESULTS: The circulating concentration of total microparticles of rats with ARDS induced by lipopolysaccharide (LPS) did not change compared with other two groups. However, ARDS rats expressed higher concentration of leukocyte- and endothelium- derived microparticles in the three groups. CONCLUSION: Our results indicate that leukocyte and endothelial cell-derived particles may play an important role in ARDS. Thus it is important not only to monitor total microparticle levels but also the phenotypes, which may contribute to the prevention and early treatment of ARDS.
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