Yu-Zheng Ge1, Ran Wu1, Hui Xin1, Hao Liu2, Tian-Ze Lu3, You-Cai Zhao4, Jiang-Wei Shen5, Zhi-Kai Hu5, Peng Yu6, Liu-Hua Zhou1, Lu-Wei Xu1, Zheng Xu1, Jian-Ping Wu1, Wen-Cheng Li1, Jia-Geng Zhu1, Rui-Peng Jia1. 1. Department of Urology, Nanjing First Hospital, Nanjing Medical University 68 Changle Road, Nanjing 210006, China ; Center for Renal Transplantation, Nanjing First Hospital, Nanjing Medical University 68 Changle Road, Nanjing 210006, China. 2. Department of Urology, The Second Affiliated Hospital of Zhejiang University School of Medicine 88 Jiefang Road, Hangzhou 310009, China. 3. Department of Urology, Nanjing First Hospital, Nanjing Medical University 68 Changle Road, Nanjing 210006, China ; Department of Pathology, Nanjing First Hospital, Nanjing Medical University 68 Changle Road, Nanjing 210006, China. 4. Department of Pathology, Nanjing First Hospital, Nanjing Medical University 68 Changle Road, Nanjing 210006, China. 5. Department of Urology, Nanjing First Hospital, Nanjing Medical University 68 Changle Road, Nanjing 210006, China ; Department of Ultrasound and Radiology, Nanjing First Hospital, Nanjing Medical University 68 Changle Road, Nanjing 210006, China. 6. Department of Urology, The First Hospital of Nanchang, Nanchang University 128 Xiangshan North Road, Nanchang 330008, China.
Abstract
BACKGROUND: Ischemic preconditioning (IPC) could protect against subsequent renal ischemia reperfusion injury (IRI). However, the mechanisms underlying IPC remain far from complete. Hence, we explored the effects of IPC on the renal and systemic hemodynamic changes, renal function and morphology, as well the involvement of endothelial and inducible nitric oxide synthase (eNOS/iNOS), and nitric oxide (NO). METHODS: Male Sprague-Dawley rats were randomly divided into five groups after right-side nephrectomy: Sham group (surgery without vascular clamping); IRI group (the left renal artery was clamped for 45 min); IPC group (pretreated with 15 min of ischemia and 10 min of reperfusion); IPC + vehicle group (administrated with 0.9% saline 5 min before IPC); and IPC + N(G)-nitro-L-arginine methylester (L-NAME) group (pretreated with L-NAME 5 min prior to IPC). The renal and systemic hemodynamic parameters, renal function and morphology, as well as eNOS, iNOS, and NO expression levels in the kidneys were measured at the indicated time points after reperfusion. RESULTS: IPC rats exhibited significant improvements in renal function, morphology, and renal artery blood flow (RABF), without obvious influence on the systemic hemodynamics and renal vein blood flow. Increased eNOS, iNOS, and NO expression levels were detected in the kidneys of IPC rats 24 h after reperfusion. Furthermore, the beneficial effects were fully abolished by the administration of L-NAME. CONCLUSIONS: The results suggest that IPC contributes to early restoration of RABF, probably through eNOS/iNOS-mediated NO production, thereby alleviating the renal dysfunction and histological damage caused by IRI.
BACKGROUND:Ischemic preconditioning (IPC) could protect against subsequent renal ischemia reperfusion injury (IRI). However, the mechanisms underlying IPC remain far from complete. Hence, we explored the effects of IPC on the renal and systemic hemodynamic changes, renal function and morphology, as well the involvement of endothelial and inducible nitric oxide synthase (eNOS/iNOS), and nitric oxide (NO). METHODS: Male Sprague-Dawley rats were randomly divided into five groups after right-side nephrectomy: Sham group (surgery without vascular clamping); IRI group (the left renal artery was clamped for 45 min); IPC group (pretreated with 15 min of ischemia and 10 min of reperfusion); IPC + vehicle group (administrated with 0.9% saline 5 min before IPC); and IPC + N(G)-nitro-L-arginine methylester (L-NAME) group (pretreated with L-NAME 5 min prior to IPC). The renal and systemic hemodynamic parameters, renal function and morphology, as well as eNOS, iNOS, and NO expression levels in the kidneys were measured at the indicated time points after reperfusion. RESULTS: IPC rats exhibited significant improvements in renal function, morphology, and renal artery blood flow (RABF), without obvious influence on the systemic hemodynamics and renal vein blood flow. Increased eNOS, iNOS, and NO expression levels were detected in the kidneys of IPC rats 24 h after reperfusion. Furthermore, the beneficial effects were fully abolished by the administration of L-NAME. CONCLUSIONS: The results suggest that IPC contributes to early restoration of RABF, probably through eNOS/iNOS-mediated NO production, thereby alleviating the renal dysfunction and histological damage caused by IRI.
Authors: Maarten G Snoeijs; Hans Vink; Niek Voesten; Maarten H Christiaans; Jan-Willem H Daemen; Arnoud G Peppelenbosch; Jan H Tordoir; Carine J Peutz-Kootstra; Wim A Buurman; Geert Willem H Schurink; L W Ernest van Heurn Journal: Am J Physiol Renal Physiol Date: 2010-09-01
Authors: Kimberley E Wever; Theo P Menting; Maroeska Rovers; J Adam van der Vliet; Gerard A Rongen; Rosalinde Masereeuw; Merel Ritskes-Hoitinga; Carlijn R Hooijmans; Michiel Warlé Journal: PLoS One Date: 2012-02-28 Impact factor: 3.240