Literature DB >> 25972537

Hepatitis B Virus-Infected HepG2hNTCP Cells Serve as a Novel Immunological Tool To Analyze the Antiviral Efficacy of CD8+ T Cells In Vitro.

Alexander Hoh1, Maximilian Heeg2, Yi Ni3, Anita Schuch4, Benedikt Binder2, Nadine Hennecke2, Hubert E Blum2, Michael Nassal2, Ulrike Protzer5, Maike Hofmann2, Stephan Urban6, Robert Thimme7.   

Abstract

CD8(+) T cells are the main effector lymphocytes in the control of hepatitis B virus (HBV) infection. However, limitations of model systems, such as low infection rates, restrict mechanistic studies of HBV-specific CD8(+) T cells. Here, we established a novel immunological cell culture model based on HBV-infected HepG2(hNTCP) cells that endogenously processed viral antigens and presented them to HBV-specific CD8(+) T cells. This induced cytolytic and noncytolytic CD8(+) T-cell effector functions and reduction of viral loads.
Copyright © 2015, American Society for Microbiology. All Rights Reserved.

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Year:  2015        PMID： 25972537      PMCID： PMC4473563          DOI： 10.1128/JVI.00605-15

Source DB:  PubMed          Journal:  J Virol        ISSN： 0022-538X            Impact factor:   5.103


  19 in total

1.  Viral clearance without destruction of infected cells during acute HBV infection.

Authors:  L G Guidotti; R Rochford; J Chung; M Shapiro; R Purcell; F V Chisari
Journal:  Science       Date:  1999-04-30       Impact factor: 47.728

Review 2.  Stealth and cunning: hepatitis B and hepatitis C viruses.

Authors:  Stefan F Wieland; Francis V Chisari
Journal:  J Virol       Date:  2005-08       Impact factor: 5.103

3.  Stable HepG2- and Huh7-based human hepatoma cell lines for efficient regulated expression of infectious hepatitis B virus.

Authors:  Dianxing Sun; Michael Nassal
Journal:  J Hepatol       Date:  2006-07-07       Impact factor: 25.083

4.  Direct ex vivo analysis of hepatitis B virus-specific CD8(+) T cells associated with the control of infection.

Authors:  M K Maini; C Boni; G S Ogg; A S King; S Reignat; C K Lee; J R Larrubia; G J Webster; A J McMichael; C Ferrari; R Williams; D Vergani; A Bertoletti
Journal:  Gastroenterology       Date:  1999-12       Impact factor: 22.682

5.  Real-time PCR quantitation of hepatitis B virus DNA using automated sample preparation and murine cytomegalovirus internal control.

Authors:  J A Garson; P R Grant; U Ayliffe; R B Ferns; R S Tedder
Journal:  J Virol Methods       Date:  2005-06       Impact factor: 2.014

Review 6.  Innate and adaptive immune responses in chronic hepatitis B virus infections: towards restoration of immune control of viral infection.

Authors:  Antonio Bertoletti; Carlo Ferrari
Journal:  Gut       Date:  2011-12-09       Impact factor: 23.059

7.  Hepatitis B and D viruses exploit sodium taurocholate co-transporting polypeptide for species-specific entry into hepatocytes.

Authors:  Yi Ni; Florian A Lempp; Stefan Mehrle; Shirin Nkongolo; Christina Kaufman; Maria Fälth; Jan Stindt; Christian Königer; Michael Nassal; Ralf Kubitz; Holger Sültmann; Stephan Urban
Journal:  Gastroenterology       Date:  2013-12-19       Impact factor: 22.682

8.  Engineering virus-specific T cells that target HBV infected hepatocytes and hepatocellular carcinoma cell lines.

Authors:  Adam J Gehring; Shao-An Xue; Zi Zong Ho; Denise Teoh; Christiane Ruedl; Adeline Chia; Sarene Koh; Seng Gee Lim; Mala K Maini; Hans Stauss; Antonio Bertoletti
Journal:  J Hepatol       Date:  2010-11-23       Impact factor: 25.083

9.  Analysis of CD8+ T-cell-mediated inhibition of hepatitis C virus replication using a novel immunological model.

Authors:  Juandy Jo; Ulrike Aichele; Nadine Kersting; Rahel Klein; Peter Aichele; Emmanuel Bisse; Andrew K Sewell; Hubert E Blum; Ralf Bartenschlager; Volker Lohmann; Robert Thimme
Journal:  Gastroenterology       Date:  2008-12-13       Impact factor: 22.682

10.  Sodium taurocholate cotransporting polypeptide is a functional receptor for human hepatitis B and D virus.

Authors:  Huan Yan; Guocai Zhong; Guangwei Xu; Wenhui He; Zhiyi Jing; Zhenchao Gao; Yi Huang; Yonghe Qi; Bo Peng; Haimin Wang; Liran Fu; Mei Song; Pan Chen; Wenqing Gao; Bijie Ren; Yinyan Sun; Tao Cai; Xiaofeng Feng; Jianhua Sui; Wenhui Li
Journal:  Elife       Date:  2012-11-13       Impact factor: 8.140
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  12 in total

1.  T cell receptor grafting allows virological control of Hepatitis B virus infection.

Authors:  Karin Wisskirchen; Janine Kah; Antje Malo; Theresa Asen; Tassilo Volz; Lena Allweiss; Jochen M Wettengel; Marc Lütgehetmann; Stephan Urban; Tanja Bauer; Maura Dandri; Ulrike Protzer
Journal:  J Clin Invest       Date:  2019-04-30       Impact factor: 14.808

2.  Disentangling molecular mechanisms regulating sensitization of interferon alpha signal transduction.

Authors:  Frédérique Kok; Marcus Rosenblatt; Melissa Teusel; Jens Timmer; Marcel Schilling; Ursula Klingmüller; Tamar Nizharadze; Vladimir Gonçalves Magalhães; Christopher Dächert; Tim Maiwald; Artyom Vlasov; Marvin Wäsch; Silvana Tyufekchieva; Katrin Hoffmann; Georg Damm; Daniel Seehofer; Tobias Boettler; Marco Binder
Journal:  Mol Syst Biol       Date:  2020-07       Impact factor: 11.429

3.  HBsAg-redirected T cells exhibit antiviral activity in HBV-infected human liver chimeric mice.

Authors:  Robert L Kruse; Thomas Shum; Haruko Tashiro; Mercedes Barzi; Zhongzhen Yi; Christina Whitten-Bauer; Xavier Legras; Beatrice Bissig-Choisat; Urtzi Garaigorta; Stephen Gottschalk; Karl-Dimiter Bissig
Journal:  Cytotherapy       Date:  2018-04-06       Impact factor: 5.414

Review 4.  Advances and Challenges in Studying Hepatitis B Virus In Vitro.

Authors:  Dvora Witt-Kehati; Maya Bitton Alaluf; Amir Shlomai
Journal:  Viruses       Date:  2016-01-14       Impact factor: 5.048

5.  Isolation and functional characterization of hepatitis B virus-specific T-cell receptors as new tools for experimental and clinical use.

Authors:  Karin Wisskirchen; Kai Metzger; Sophia Schreiber; Theresa Asen; Luise Weigand; Christina Dargel; Klaus Witter; Elisa Kieback; Martin F Sprinzl; Wolfgang Uckert; Matthias Schiemann; Dirk H Busch; Angela M Krackhardt; Ulrike Protzer
Journal:  PLoS One       Date:  2017-08-08       Impact factor: 3.240

Review 6.  Hepatitis B Virus Adaptation to the CD8+ T Cell Response: Consequences for Host and Pathogen.

Authors:  Sheila F Lumley; Anna L McNaughton; Paul Klenerman; Katrina A Lythgoe; Philippa C Matthews
Journal:  Front Immunol       Date:  2018-07-16       Impact factor: 7.561

7.  Spatiotemporal Differences in Presentation of CD8 T Cell Epitopes during Hepatitis B Virus Infection.

Authors:  Atefeh Khakpoor; Yi Ni; Antony Chen; Zi Zong Ho; Vincent Oei; Ninghan Yang; Reshmi Giri; Jia Xin Chow; Anthony T Tan; Patrick T Kennedy; Mala Maini; Stephan Urban; Antonio Bertoletti
Journal:  J Virol       Date:  2019-02-05       Impact factor: 5.103

8.  CRISPR/Cas9 nickase-mediated disruption of hepatitis B virus open reading frame S and X.

Authors:  Madina Karimova; Niklas Beschorner; Werner Dammermann; Jan Chemnitz; Daniela Indenbirken; Jan-Hendrik Bockmann; Adam Grundhoff; Stefan Lüth; Frank Buchholz; Julian Schulze zur Wiesch; Joachim Hauber
Journal:  Sci Rep       Date:  2015-09-03       Impact factor: 4.379

Review 9.  Viral hepatitis and liver cancer.

Authors:  Marc Ringelhan; Jane A McKeating; Ulrike Protzer
Journal:  Philos Trans R Soc Lond B Biol Sci       Date:  2017-10-19       Impact factor: 6.237

Review 10.  CRISPR-Cas Targeting of Host Genes as an Antiviral Strategy.

Authors:  Shuliang Chen; Xiao Yu; Deyin Guo
Journal:  Viruses       Date:  2018-01-16       Impact factor: 5.048
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