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Literature DB >> 25971974

The tRNA Splicing Endonuclease Complex Cleaves the Mitochondria-localized CBP1 mRNA.

Tatsuhisa Tsuboi¹, Reina Yamazaki¹, Risa Nobuta¹, Ken Ikeuchi¹, Shiho Makino¹, Ayumi Ohtaki¹, Yutaka Suzuki², Tohru Yoshihisa³, Christopher Trotta⁴, Toshifumi Inada⁵.

Abstract

The tRNA splicing endonuclease (Sen) complex is located on the mitochondrial outer membrane and splices precursor tRNAs in Saccharomyces cerevisiae. Here, we demonstrate that the Sen complex cleaves the mitochondria-localized mRNA encoding Cbp1 (cytochrome b mRNA processing 1). Endonucleolytic cleavage of this mRNA required two cis-elements: the mitochondrial targeting signal and the stem-loop 652-726-nt region. Mitochondrial localization of the Sen complex was required for cleavage of the CBP1 mRNA, and the Sen complex cleaved this mRNA directly in vitro. We propose that the Sen complex cleaves the CBP1 mRNA, which is co-translationally localized to mitochondria via its mitochondrial targeting signal.

Entities: Gene Species

Keywords: endonuclease; mRNA decay; mRNA decay, endonuclease; mRNA localization; mitochondria; quality control; tRNA splicing; transfer RNA (tRNA); translation

Mesh：

Substances：

Year: 2015 PMID： 25971974 PMCID： PMC4481206 DOI： 10.1074/jbc.M114.634592

Source DB: PubMed Journal: J Biol Chem ISSN： 0021-9258 Impact factor: 5.157

31 in total

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Authors: Antonia M G van den Elzen; Julien Henri; Noureddine Lazar; María Eugenia Gas; Dominique Durand; François Lacroute; Magali Nicaise; Herman van Tilbeurgh; Bertrand Séraphin; Marc Graille
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4. Cleavage of pre-tRNAs by the splicing endonuclease requires a composite active site.

Authors: Christopher R Trotta; Sergey V Paushkin; Meenal Patel; Hong Li; Stuart W Peltz
Journal: Nature Date: 2006-05-18 Impact factor: 49.962

5. Endonucleolytic cleavage of eukaryotic mRNAs with stalls in translation elongation.

Authors: Meenakshi K Doma; Roy Parker
Journal: Nature Date: 2006-03-23 Impact factor: 49.962

6. New yeast-Escherichia coli shuttle vectors constructed with in vitro mutagenized yeast genes lacking six-base pair restriction sites.

Authors: R D Gietz; A Sugino
Journal: Gene Date: 1988-12-30 Impact factor: 3.688

7. Three new dominant drug resistance cassettes for gene disruption in Saccharomyces cerevisiae.

Authors: A L Goldstein; J H McCusker
Journal: Yeast Date: 1999-10 Impact factor: 3.239

8. Cbp1 is required for translation of the mitochondrial cytochrome b mRNA of Saccharomyces cerevisiae.

Authors: Maria A Islas-Osuna; Timothy P Ellis; Lorraine L Marnell; Telsa M Mittelmeier; Carol L Dieckmann
Journal: J Biol Chem Date: 2002-07-30 Impact factor: 5.157

9. Identification of a human endonuclease complex reveals a link between tRNA splicing and pre-mRNA 3' end formation.

Authors: Sergey V Paushkin; Meenal Patel; Bansri S Furia; Stuart W Peltz; Christopher R Trotta
Journal: Cell Date: 2004-04-30 Impact factor: 41.582

10. Possibility of cytoplasmic pre-tRNA splicing: the yeast tRNA splicing endonuclease mainly localizes on the mitochondria.

Authors: Tohru Yoshihisa; Kaori Yunoki-Esaki; Chie Ohshima; Nobuyuki Tanaka; Toshiya Endo
Journal: Mol Biol Cell Date: 2003-05-03 Impact factor: 4.138

10 in total

1. Comparative parallel analysis of RNA ends identifies mRNA substrates of a tRNA splicing endonuclease-initiated mRNA decay pathway.

Authors: Jennifer E Hurtig; Michelle A Steiger; Vinay K Nagarajan; Tao Li; Ti-Chun Chao; Kuang-Lei Tsai; Ambro van Hoof
Journal: Proc Natl Acad Sci U S A Date: 2021-03-09 Impact factor: 11.205

2. Yeast Dxo1 is required for 25S rRNA maturation and acts as a transcriptome-wide distributive exonuclease.

Authors: Jennifer E Hurtig; Ambro van Hoof
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Review 3. Recent insights into the structure, function, and regulation of the eukaryotic transfer RNA splicing endonuclease complex.

Authors: Cassandra K Hayne; Tanae A Lewis; Robin E Stanley
Journal: Wiley Interdiscip Rev RNA Date: 2022-02-14 Impact factor: 9.349

The tRNA Splicing Endonuclease Complex Cleaves the Mitochondria-localized CBP1 mRNA.

1. Mfold web server for nucleic acid folding and hybridization prediction.

2. Kinetic analysis reveals the ordered coupling of translation termination and ribosome recycling in yeast.

3. Dissection of Dom34-Hbs1 reveals independent functions in two RNA quality control pathways.

4. Cleavage of pre-tRNAs by the splicing endonuclease requires a composite active site.

5. Endonucleolytic cleavage of eukaryotic mRNAs with stalls in translation elongation.

6. New yeast-Escherichia coli shuttle vectors constructed with in vitro mutagenized yeast genes lacking six-base pair restriction sites.

7. Three new dominant drug resistance cassettes for gene disruption in Saccharomyces cerevisiae.

8. Cbp1 is required for translation of the mitochondrial cytochrome b mRNA of Saccharomyces cerevisiae.

9. Identification of a human endonuclease complex reveals a link between tRNA splicing and pre-mRNA 3' end formation.

10. Possibility of cytoplasmic pre-tRNA splicing: the yeast tRNA splicing endonuclease mainly localizes on the mitochondria.

1. Comparative parallel analysis of RNA ends identifies mRNA substrates of a tRNA splicing endonuclease-initiated mRNA decay pathway.

2. Yeast Dxo1 is required for 25S rRNA maturation and acts as a transcriptome-wide distributive exonuclease.

Review 3. Recent insights into the structure, function, and regulation of the eukaryotic transfer RNA splicing endonuclease complex.

Review 4. tRNA dynamics between the nucleus, cytoplasm and mitochondrial surface: Location, location, location.

5. Suppressors of mRNA Decapping Defects Restore Growth Without Major Effects on mRNA Decay Rates or Abundance.

6. From powerhouse to processing plant: conserved roles of mitochondrial outer membrane proteins in tRNA splicing.

Review 7. tRNA Processing and Subcellular Trafficking Proteins Multitask in Pathways for Other RNAs.

8. Genetic bypass of essential RNA repair enzymes in budding yeast.

9. A protein quality control pathway at the mitochondrial outer membrane.

10. Quality controls induced by aberrant translation.