| Literature DB >> 25971910 |
Di Wu1,2, Michael P Gantier3,4.
Abstract
microRNA (miRNA) microarray normalization is a critical step for the identification of truly differentially expressed miRNAs. This is particularly important when dealing with cancer samples that have a global miRNA decrease. In this chapter, we provide a simple step-by-step procedure that can be used to normalize Affymetrix miRNA microarrays, relying on robust normal-exponential background correction with cyclic loess normalization.Entities:
Keywords: Affymetrix; Cancer samples; Normalization; miRNA microarray; microRNA
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Year: 2016 PMID： 25971910 DOI： 10.1007/7651_2015_239
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745