Antonio J López-Farré1, Juana María Santos-Sancho2, Javier Modrego3, Antonio Segura4, José J Zamorano-León3, Leyre Martín5, Ana Sánchez-Fructuoso5, Pablo Rodríguez-Sierra3, Fernando Prados6, Alonso Mateos7, José Herrero5, Francisco del Río8, Alberto Barrientos9. 1. Medicine Department, Universidad Complutense de Madrid, Madrid, Spain. 2. Preventive Medicine and Public Health, School of Medicine, Universidad Complutense de Madrid, Madrid, Spain. 3. Instituto de Investigación Sanitaria del Hospital Clínico San Carlos (IdISSC), Madrid, Spain. 4. Health Sciences Institute, Talavera de la Reina, Toledo, Spain. 5. Nephrology Department, Hospital Clínico San Carlos, Universidad Complutense de Madrid, C/Profesor Martín Lagos s/n, Madrid, 28040, Spain. 6. Servicio de Atención Médica Urgente (SAMUR)-Protección Civil, Madrid, Spain. 7. Servicio de Urgencia Médica de Madrid (SUMMA 112), Madrid, Spain. 8. Transplant Coordination Unit, Hospital Clínico San Carlos, Medicine Department, School of Medicine, Universidad Complutense de Madrid, C/Profesor Martín Lagos s/n, Madrid, 28040, Spain. 9. Nephrology Department, Hospital Clínico San Carlos, Universidad Complutense de Madrid, C/Profesor Martín Lagos s/n, Madrid, 28040, Spain. dr.albertobarrientos@gmail.com.
Abstract
BACKGROUND/AIMS: Kidneys from uncontrolled non heart-beating donors achieve a good level of renal function after transplantation. However, a number of them will never function in the recipient. Our aim was to determine if serum biomarkers associated with platelet activity, inflammation and the nitric oxide system in uncontrolled non heart-beating donors may help to predict no renal function recovery after renal transplantation. METHODS: Serum levels of interleukin (IL)-6, IL-10, intercellular cell adhesion molecule-1 (ICAM-1), cyclic guanosine monophosphate (cGMP), nitrite + nitrate and platelet factor-4 (PF4) were measured using enzyme-linked immunosorbent assay (ELISA) kits in 88 uncontrolled non heart-beating donors divided according to the renal functionality achieved in the recipients into functional (n = 76) and non functional (n = 12). RESULTS: Kidneys from donors with higher IL-6 levels (>900 pg/ml) were functional after transplantation. Serum cGMP levels below 372.3 fmol/l were also associated with kidneys that recovered the renal function. However, serum levels of PF4 showed the best correlation with recovery of renal functional in the recipients since they were significantly lower in the donors whose kidneys functioned after transplantation. CONCLUSIONS: Serum PF4 levels in uncontrolled non heart-beating donors may be a good predictor for kidneys that never will reach functional recovery. Some serum cGMP, IL-6 and IL-10 levels may simply help identify kidneys that will function after transplantation.
BACKGROUND/AIMS: Kidneys from uncontrolled non heart-beating donors achieve a good level of renal function after transplantation. However, a number of them will never function in the recipient. Our aim was to determine if serum biomarkers associated with platelet activity, inflammation and the nitric oxide system in uncontrolled non heart-beating donors may help to predict no renal function recovery after renal transplantation. METHODS: Serum levels of interleukin (IL)-6, IL-10, intercellular cell adhesion molecule-1 (ICAM-1), cyclic guanosine monophosphate (cGMP), nitrite + nitrate and platelet factor-4 (PF4) were measured using enzyme-linked immunosorbent assay (ELISA) kits in 88 uncontrolled non heart-beating donors divided according to the renal functionality achieved in the recipients into functional (n = 76) and non functional (n = 12). RESULTS: Kidneys from donors with higher IL-6 levels (>900 pg/ml) were functional after transplantation. Serum cGMP levels below 372.3 fmol/l were also associated with kidneys that recovered the renal function. However, serum levels of PF4 showed the best correlation with recovery of renal functional in the recipients since they were significantly lower in the donors whose kidneys functioned after transplantation. CONCLUSIONS: Serum PF4 levels in uncontrolled non heart-beating donors may be a good predictor for kidneys that never will reach functional recovery. Some serum cGMP, IL-6 and IL-10 levels may simply help identify kidneys that will function after transplantation.
Authors: Stephanie Pitsilos; Jennifer Hunt; Emile R Mohler; Anand M Prabhakar; Mortimer Poncz; Jennine Dawicki; Tigran Z Khalapyan; Megan L Wolfe; Ronald Fairman; Marc Mitchell; Jeffrey Carpenter; Michael A Golden; Douglas B Cines; Bruce S Sachais Journal: Thromb Haemost Date: 2003-12 Impact factor: 5.249
Authors: Anja Haase-Fielitz; Rinaldo Bellomo; Prasad Devarajan; David Story; George Matalanis; Duska Dragun; Michael Haase Journal: Crit Care Med Date: 2009-02 Impact factor: 7.598