Literature DB >> 2597108

Human serum amyloid A protein. Behaviour in aqueous and urea-containing solutions and antibody production.

A F Strachan1, E G Shephard, D U Bellstedt, G A Coetzee, D R van der Westhuyzen, F C de Beer.   

Abstract

Human serum amyloid A protein (apo-SAA) can be prepared by gel filtration of delipidated acute-phase high-density lipoprotein in the presence of urea. The resultant apo-SAA is soluble (greater than 90% solubility) in a wide range of buffer solutions, with all of the six major isoforms of apo-SAA being equally soluble. In urea-containing solutions the isoforms behave qualitatively differently in various urea concentrations, probably reflecting subtle primary-structure variations. The higher-pI isoforms are only completely unfolded at greater than 7 M-urea. By immunizing with apo-SAA adsorbed to acid-treated bacteria (Salmonella minnesota R595), high-titre antibodies can easily be elicited in rabbits.

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Year:  1989        PMID： 2597108      PMCID： PMC1133438          DOI： 10.1042/bj2630365

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  23 in total

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Authors:  O H LOWRY; N J ROSEBROUGH; A L FARR; R J RANDALL
Journal:  J Biol Chem       Date:  1951-11       Impact factor: 5.157

2.  Acid-treated, naked bacteria as immune carriers for protein antigens.

Authors:  D U Bellstedt; P A Human; G F Rowland; K J Van der Merwe
Journal:  J Immunol Methods       Date:  1987-04-16       Impact factor: 2.303

3.  On the subunit structure of the protein of human serum high density lipoprotein. I. A study of its major polypeptide component (Sephadex, fraction 3).

Authors:  C Edelstein; C T Lim; A M Scanu
Journal:  J Biol Chem       Date:  1972-09-25       Impact factor: 5.157

4.  [A new laboratory fermentor for the cultivation of microorganisms by means of turbidostatical, chemostatical and "batch" methods. I. Technical description of the fermentor].

Authors:  S Schlecht; K Ring; J Kutscher; W Eschweiler
Journal:  Zentralbl Bakteriol Orig       Date:  1968-03

5.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

6.  Identification of three isoform patterns of human serum amyloid A protein.

Authors:  A F Strachan; F C de Beer; D R van der Westhuyzen; G A Coetzee
Journal:  Biochem J       Date:  1988-02-15       Impact factor: 3.857

7.  Standardisation of the quantitation of serum amyloid A protein (SAA) in human serum.

Authors:  N L Godenir; M S Jeenah; G A Coetzee; D R Van der Westhuyzen; A F Strachan; F C De Beer
Journal:  J Immunol Methods       Date:  1985-11-07       Impact factor: 2.303

8.  Human serum amyloid A. Three hepatic mRNAs and the corresponding proteins in one person.

Authors:  B Kluve-Beckerman; F E Dwulet; M D Benson
Journal:  J Clin Invest       Date:  1988-11       Impact factor: 14.808

9.  Production of an amino acid sequence-specific antiserum against human amyloid A (AA) and serum amyloid A (SAA) protein.

Authors:  A Grubb; H Löfberg; H Thysell; L Ljunggren; T Olsson; M Skinner; T Shirahama; A S Cohen
Journal:  Scand J Clin Lab Invest       Date:  1987-10       Impact factor: 1.713

10.  Amyloidogenesis. One serum amyloid A isotype is selectively removed from the circulation.

Authors:  R L Meek; J S Hoffman; E P Benditt
Journal:  J Exp Med       Date:  1986-03-01       Impact factor: 14.307
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  11 in total

1.  Mouse serum amyloid A protein. Complete amino acid sequence and mRNA analysis of a new isoform.

Authors:  M C de Beer; F C de Beer; C M Beach; I Carreras; J D Sipe
Journal:  Biochem J       Date:  1992-05-01       Impact factor: 3.857

2.  SAA does not induce cytokine production in physiological conditions.

Authors:  Myung-Hee Kim; Maria C de Beer; Joanne M Wroblewski; Nancy R Webb; Frederick C de Beer
Journal:  Cytokine       Date:  2012-11-17       Impact factor: 3.861

3.  Serum Amyloid A Is an Exchangeable Apolipoprotein.

Authors:  Patricia G Wilson; Joel C Thompson; Preetha Shridas; Patrick J McNamara; Maria C de Beer; Frederick C de Beer; Nancy R Webb; Lisa R Tannock
Journal:  Arterioscler Thromb Vasc Biol       Date:  2018-08       Impact factor: 8.311

4.  High-density lipoprotein inhibits serum amyloid A-mediated reactive oxygen species generation and NLRP3 inflammasome activation.

Authors:  Preetha Shridas; Maria C De Beer; Nancy R Webb
Journal:  J Biol Chem       Date:  2018-07-05       Impact factor: 5.157

5.  Anti-inflammatory HDL becomes pro-inflammatory during the acute phase response. Loss of protective effect of HDL against LDL oxidation in aortic wall cell cocultures.

Authors:  B J Van Lenten; S Y Hama; F C de Beer; D M Stafforini; T M McIntyre; S M Prescott; B N La Du; A M Fogelman; M Navab
Journal:  J Clin Invest       Date:  1995-12       Impact factor: 14.808

6.  Impact of serum amyloid A on high density lipoprotein composition and levels.

Authors:  Maria C de Beer; Nancy R Webb; Joanne M Wroblewski; Victoria P Noffsinger; Debra L Rateri; Ailing Ji; Deneys R van der Westhuyzen; Frederick C de Beer
Journal:  J Lipid Res       Date:  2010-07-27       Impact factor: 5.922

7.  Serum amyloid A protein enhances the activity of secretory non-pancreatic phospholipase A2.

Authors:  W Pruzanski; F C de Beer; M C de Beer; E Stefanski; P Vadas
Journal:  Biochem J       Date:  1995-07-15       Impact factor: 3.857

8.  Pathogenic serum amyloid A 1.1 shows a long oligomer-rich fibrillation lag phase contrary to the highly amyloidogenic non-pathogenic SAA2.2.

Authors:  Saipraveen Srinivasan; Sanket Patke; Yun Wang; Zhuqiu Ye; Jeffrey Litt; Sunit K Srivastava; Maria M Lopez; Dmitry Kurouski; Igor K Lednev; Ravi S Kane; Wilfredo Colón
Journal:  J Biol Chem       Date:  2012-12-05       Impact factor: 5.157

9.  Expression of recombinant human serum amyloid A in mammalian cells and demonstration of the region necessary for high-density lipoprotein binding and amyloid fibril formation by site-directed mutagenesis.

Authors:  H Patel; J Bramall; H Waters; M C De Beer; P Woo
Journal:  Biochem J       Date:  1996-09-15       Impact factor: 3.857

10.  Investigation of the solubility and the potentials for purification of serum amyloid A (SAA) from equine acute phase serum--a pilot study.

Authors:  Michelle B Christensen; Jens Christian Sørensen; Stine Jacobsen; Mads Kjelgaard-Hansen
Journal:  BMC Res Notes       Date:  2013-04-16
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