| Literature DB >> 25970271 |
Yanan Zhu, Hao Yu, Wei Wang, Xiaohua Gong, Ke Yao.
Abstract
Entities:
Year: 2015 PMID: 25970271 PMCID: PMC4430210 DOI: 10.1371/journal.pone.0125949
Source DB: PubMed Journal: PLoS One ISSN: 1932-6203 Impact factor: 3.240
Fig 4Statistical analysis of DAPI (A) and PI (B) loading in different solutions.
Data are presented as mean±SDs. There was a significant difference in the percentage of dye-stained cells between the Cx50WILD and Cx50V44A groups in the Ca2+-free environment (P<0.001, marked with ***); there was also a significant difference between the Ca2+-free Cx50WILD and the 1.2 mM Ca2+/300 μM FFA Cx50WILD group (P<0.001, marked with ***).
Fig 5PI dye uptake in HeLa cells stably transfected with Cx50WILD and Cx50V44A.
The PI dye uptake assay revealed similar results as the DAPI dye uptake assay. The three pictures in the last line show the Cx50 protein, PI-stained nuclei (nucleoli)/cytoplasm, and merged look of the wild-type cells in Ca2+-free HBSS. Scale bar: 20 μm.