Seyed Reza Mirhafez1, Amir Avan2, Alireza Pasdar3, Elaheh Kazemi4, Faezeh Ghasemi5, Amir Tajbakhsh5, Samaneh Tabaee6, Gordon A Ferns7, Majid Ghayour-Mobarhan8. 1. Department of Basic Medical Sciences, Neyshabur University of Medical Sciences, Neyshabur, Iran; Department of New Science and Technologies, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran. 2. Department of New Science and Technologies, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran; Biochemistry of Nutrition Research Center, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran. 3. Department of New Science and Technologies, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran; Division of Applied Medicine, Medical School, University of Aberdeen, Foresterhill, Aberdeen AB25 2ZD, UK. 4. Department of Biochemistry, International Campus of Shahid Sadoughi University of Medical Sciences, Yazd, Iran. 5. Department of New Science and Technologies, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran. 6. Department of Basic Medical Sciences, Neyshabur University of Medical Sciences, Neyshabur, Iran. 7. Brighton & Sussex Medical School, Division of Medical Education, Falmer, Brighton, Sussex BN1 9PH, UK. 8. Biochemistry of Nutrition Research Center, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran. Electronic address: ghayourm@mums.ac.ir.
Abstract
BACKGROUND: The G-308A single nucleotide polymorphism (SNP) in the promoter of tumor necrosis factor-alpha (TNF-α) gene has previously been reported to be associated with cardiovascular risk. However the potential association of this polymorphism with metabolic syndrome (MetS) is unclear. The aim of the current study was to explore the association of this TNF-α gene polymorphism with MetS in an Iranian population. METHODS/PATIENTS: Two hundred and twenty two subjects were recruited and anthropometric and biochemical parameters were determined. Genotyping was performed using the amplification refractory mutation system polymerase chain reaction. The association of the genetic-polymorphism with MetS was evaluated by univariate and multivariate analyses. RESULTS: MetS subjects had a significantly (P<0.05) higher level of fasted serum triglycerides, body-mass-index, waist-circumference, blood pressure and fasting-blood-glucose, and lower level of high-density lipoprotein cholesterol. The possession of AA or GA genotype of the TNF-α gene was not associated with MetS in our population. However the AA genotype of TNF-α was related to an increased level of triglyceride in MetS patients, compared to the control group. CONCLUSION: TNF-α G-308A polymorphism is unlikely to play an important role in the development of MetS in our population.
BACKGROUND: The G-308A single nucleotide polymorphism (SNP) in the promoter of tumor necrosis factor-alpha (TNF-α) gene has previously been reported to be associated with cardiovascular risk. However the potential association of this polymorphism with metabolic syndrome (MetS) is unclear. The aim of the current study was to explore the association of this TNF-α gene polymorphism with MetS in an Iranian population. METHODS/PATIENTS: Two hundred and twenty two subjects were recruited and anthropometric and biochemical parameters were determined. Genotyping was performed using the amplification refractory mutation system polymerase chain reaction. The association of the genetic-polymorphism with MetS was evaluated by univariate and multivariate analyses. RESULTS: MetS subjects had a significantly (P<0.05) higher level of fasted serum triglycerides, body-mass-index, waist-circumference, blood pressure and fasting-blood-glucose, and lower level of high-density lipoprotein cholesterol. The possession of AA or GA genotype of the TNF-α gene was not associated with MetS in our population. However the AA genotype of TNF-α was related to an increased level of triglyceride in MetS patients, compared to the control group. CONCLUSION: TNF-α G-308A polymorphism is unlikely to play an important role in the development of MetS in our population.
Authors: Seyed Reza Mirhafez; Amir Avan; Alireza Pasdar; Sara Khatamianfar; Leila Hosseinzadeh; Shiva Ganjali; Ali Movahedi; Maryam Pirhoushiaran; Valentina Gómez Mellado; Domenico Rosace; Anne van Krieken; Mahdi Nohtani; Gordon A Ferns; Majid Ghayour-Mobarhan Journal: Int J Mol Cell Med Date: 2016