Literature DB >> 25966643

Assessment of four protocols for rapid bacterial identification from positive blood culture pellets by matrix-assisted laser desorption ionization-time of flight mass spectrometry (Vitek® MS).

Jean Thomin1, Guillaume Ghislain Aubin2, Fabrice Foubert3, Stéphane Corvec4.   

Abstract

In this study, we developed and compared four protocols to prepare a bacterial pellet from 944 positive blood cultures for direct MALDI-TOF mass spectrometry Vitek® MS analysis. Protocol 4, tested on 200 monomicrobial samples, allowed 83% of bacterial identification. This easy, fast, cheap and accurate method is promising in daily practice, especially to limit broad range antibiotic treatment.
Copyright © 2015 Elsevier B.V. All rights reserved.

Keywords:  Blood bottle culture; MALDI-TOF; Rapid identification

Mesh：

Year:  2015        PMID： 25966643     DOI： 10.1016/j.mimet.2015.05.010

Source DB:  PubMed          Journal:  J Microbiol Methods        ISSN： 0167-7012            Impact factor:   2.363


  8 in total

1.  Performance of microbial identification by MALDI-TOF MS and susceptibility testing by VITEK 2 from positive blood cultures after minimal incubation on solid media.

Authors:  S L Mitchell; K Alby
Journal:  Eur J Clin Microbiol Infect Dis       Date:  2017-06-28       Impact factor: 3.267

2.  Direct Identification of 80 Percent of Bacteria from Blood Culture Bottles by Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry Using a 10-Minute Extraction Protocol.

Authors:  Loïc Simon; Estelle Ughetto; Alice Gaudart; Nicolas Degand; Romain Lotte; Raymond Ruimy
Journal:  J Clin Microbiol       Date:  2019-01-30       Impact factor: 5.948

3.  Improved blood culture workflow for faster identification of KPC-producing Enterobacterales.

Authors:  Bruna Mara Silva Seco; Juliana Coutinho Campos; Darlan Augusto da Costa Rocha; Aline Valerio de Lima; Fernanda Filomena de Oliveira; Mara Elisa Borsato Lemo; Suely Carlos Ferreira Sampaio; Jorge Luiz Mello Sampaio
Journal:  Braz J Microbiol       Date:  2018-12-06       Impact factor: 2.476

Review 4.  Clinical and Biological Features of Cutibacterium (Formerly Propionibacterium) avidum, an Underrecognized Microorganism.

Authors:  Stéphane Corvec
Journal:  Clin Microbiol Rev       Date:  2018-05-30       Impact factor: 26.132

5.  Misidentification of Cutibacterium namnetense as Cutibacterium acnes among clinical isolates by MALDI-TOF VitekMS: usefulness of gyrB sequencing and new player in bone infections.

Authors:  L Ruffier d'Epenoux; N Arshad; P Bémer; M-E Juvin; G Le Gargasson; A Guillouzouic; S Corvec
Journal:  Eur J Clin Microbiol Infect Dis       Date:  2020-05-07       Impact factor: 3.267

6.  Unusual Case of Spondylodiscitis due to Listeria monocytogenes.

Authors:  Guillaume Ghislain Aubin; David Boutoille; Romain Bourcier; Jocelyne Caillon; Didier Lepelletier; Pascale Bémer; Stéphane Corvec
Journal:  J Bone Jt Infect       Date:  2016-02-29

7.  Multicenter assessment of the rapid Unyvero Blood Culture molecular assay.

Authors:  Sandra Christina Burrack-Lange; Yoann Personne; Monika Huber; Elisabeth Winkler; Jan Weile; Cornelius Knabbe; Julia Görig; Holger Rohde
Journal:  J Med Microbiol       Date:  2018-07-27       Impact factor: 2.472

8.  Rapid detection of carbapenemase activity of Enterobacteriaceae isolated from positive blood cultures by MALDI-TOF MS.

Authors:  Jiajia Yu; Jingxian Liu; Yuanrui Li; Jing Yu; Weinan Zhu; Ying Liu; Lisong Shen
Journal:  Ann Clin Microbiol Antimicrob       Date:  2018-05-18       Impact factor: 3.944
  8 in total

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