Takhar Kasumov1, Thomas P J Solomon2, Calvin Hwang2, Hazel Huang2, Jacob M Haus2, Renliang Zhang3,4, John P Kirwan1,2,5,6. 1. Department of Gastroenterology & Hepatology, Cleveland Clinic, Cleveland, Ohio, USA. 2. Department of Pathobiology, Cleveland Clinic, Cleveland, Ohio, USA. 3. Department of Cardiovascular Medicine, Cleveland Clinic, Cleveland, Ohio, USA. 4. Center for Cardiovascular Diagnostics and Prevention, Cleveland Clinic, Cleveland, Ohio, USA. 5. Department of Nutrition, Case Western Reserve, University School of Medicine, Cleveland, Ohio, USA. 6. Metabolic Translational Research Center, Endocrine and Metabolism Institute, Cleveland Clinic, Cleveland, Ohio, USA.
Abstract
OBJECTIVE: To assess the effect of exercise training on insulin sensitivity and plasma ceramides in obesity and type 2 diabetes (T2D). METHODS: Twenty-four adults with obesity and normal glucose tolerance (NGT, n = 14) or diabetes (n = 10) were studied before and after a 12-week supervised exercise-training program (5 days/week, 1 h/day, 80-85% of maximum heart rate). Changes in body composition were assessed using hydrostatic weighing and computed tomography. Peripheral tissue insulin sensitivity was assessed by a 40 mU/m(2) /min hyperinsulinemic euglycemic clamp. Plasma ceramides (C14:0, C16:0, C18:0, C18:1, C20:0, C24:0, and C24:1) were quantified using electrospray ionization tandem mass spectrometry after separation with HPLC. RESULTS: Plasma ceramides were similar for the subjects with obesity and NGT and the subjects with diabetes, despite differences in glucose tolerance. Exercise significantly reduced body weight and adiposity and increased peripheral insulin sensitivity in both groups (P < 0.05). In addition, plasma C14:0, C16:0, C18:1, and C24:0 ceramide levels were reduced in all subjects following the intervention (P < 0.05). Decreases in total (r = -0.51, P = 0.02) and C14:0 (r = -0.56, P = 0.009) ceramide were negatively correlated with the increase in insulin sensitivity. CONCLUSIONS: Ceramides are linked to exercise training-induced improvements in insulin sensitivity, and plasma C14:0 ceramide may provide a specific target for investigating lipid-related insulin resistance in obesity and T2D.
OBJECTIVE: To assess the effect of exercise training on insulin sensitivity and plasma ceramides in obesity and type 2 diabetes (T2D). METHODS: Twenty-four adults with obesity and normal glucose tolerance (NGT, n = 14) or diabetes (n = 10) were studied before and after a 12-week supervised exercise-training program (5 days/week, 1 h/day, 80-85% of maximum heart rate). Changes in body composition were assessed using hydrostatic weighing and computed tomography. Peripheral tissue insulin sensitivity was assessed by a 40 mU/m(2) /min hyperinsulinemic euglycemic clamp. Plasma ceramides (C14:0, C16:0, C18:0, C18:1, C20:0, C24:0, and C24:1) were quantified using electrospray ionization tandem mass spectrometry after separation with HPLC. RESULTS: Plasma ceramides were similar for the subjects with obesity and NGT and the subjects with diabetes, despite differences in glucose tolerance. Exercise significantly reduced body weight and adiposity and increased peripheral insulin sensitivity in both groups (P < 0.05). In addition, plasma C14:0, C16:0, C18:1, and C24:0 ceramide levels were reduced in all subjects following the intervention (P < 0.05). Decreases in total (r = -0.51, P = 0.02) and C14:0 (r = -0.56, P = 0.009) ceramide were negatively correlated with the increase in insulin sensitivity. CONCLUSIONS:Ceramides are linked to exercise training-induced improvements in insulin sensitivity, and plasma C14:0 ceramide may provide a specific target for investigating lipid-related insulin resistance in obesity and T2D.
Authors: John J Dubé; Francesca Amati; Maja Stefanovic-Racic; Frederico G S Toledo; Sarah E Sauers; Bret H Goodpaster Journal: Am J Physiol Endocrinol Metab Date: 2008-03-04 Impact factor: 4.310
Authors: Maria Kolak; Jukka Westerbacka; Vidya R Velagapudi; Dick Wågsäter; Laxman Yetukuri; Janne Makkonen; Aila Rissanen; Anna-Maija Häkkinen; Monica Lindell; Robert Bergholm; Anders Hamsten; Per Eriksson; Rachel M Fisher; Matej Oresic; Hannele Yki-Järvinen Journal: Diabetes Date: 2007-07-09 Impact factor: 9.461
Authors: Thomas P J Solomon; Sakita N Sistrun; Raj K Krishnan; Luis F Del Aguila; Christine M Marchetti; Susan M O'Carroll; Valerie B O'Leary; John P Kirwan Journal: J Appl Physiol (1985) Date: 2008-03-06
Authors: Jacob M Haus; Sangeeta R Kashyap; Takhar Kasumov; Renliang Zhang; Karen R Kelly; Ralph A Defronzo; John P Kirwan Journal: Diabetes Date: 2008-11-13 Impact factor: 9.461
Authors: Bryan C Bergman; Joseph T Brozinick; Allison Strauss; Samantha Bacon; Anna Kerege; Hai Hoang Bui; Phil Sanders; Parker Siddall; Ming Shang Kuo; Leigh Perreault Journal: Am J Physiol Endocrinol Metab Date: 2015-06-30 Impact factor: 4.310
Authors: Bianca Williams; Jason Correnti; Amanke Oranu; Annie Lin; Victoria Scott; Maxine Annoh; James Beck; Emma Furth; Victoria Mitchell; Can E Senkal; Lina Obeid; Rotonya M Carr Journal: FASEB J Date: 2017-09-01 Impact factor: 5.191
Authors: Jacqueline Stöckli; Kelsey H Fisher-Wellman; Rima Chaudhuri; Xiao-Yi Zeng; Daniel J Fazakerley; Christopher C Meoli; Kristen C Thomas; Nolan J Hoffman; Salvatore P Mangiafico; Chrysovalantou E Xirouchaki; Chieh-Hsin Yang; Olga Ilkayeva; Kari Wong; Gregory J Cooney; Sofianos Andrikopoulos; Deborah M Muoio; David E James Journal: J Biol Chem Date: 2017-10-05 Impact factor: 5.157
Authors: Lisa M Butler; Peter J Meikle; Lisa G Horvath; Hui-Ming Lin; Kevin Huynh; Manish Kohli; Winston Tan; Arun A Azad; Nicole Yeung; Kate L Mahon; Blossom Mak; Peter D Sutherland; Andrew Shepherd; Natalie Mellett; Maria Docanto; Corey Giles; Margaret M Centenera Journal: Prostate Cancer Prostatic Dis Date: 2021-03-21 Impact factor: 5.554
Authors: Julian M Gaitán; Hyo Youl Moon; Matthew Stremlau; Dena B Dubal; Dane B Cook; Ozioma C Okonkwo; Henriette van Praag Journal: Front Endocrinol (Lausanne) Date: 2021-05-20 Impact factor: 5.555