Sebastian Hinz1, Jürgen Tepel2, Christian Röder3, Holger Kalthoff3, Thomas Becker4. 1. Department of General and Thoracic Surgery, University Hospital Schleswig-Holstein, Kiel, Germany sebastian.hinz@uksh-kiel.de. 2. Clinic for General, Thoracic and Visceral Surgery, Klinikum Osnabrück, Osnabrück, Germany. 3. Institute for Experimental Cancer Research, Cancer Center North, University Hospital Schleswig-Holstein, Kiel, Germany. 4. Department of General and Thoracic Surgery, University Hospital Schleswig-Holstein, Kiel, Germany.
Abstract
BACKGROUND/AIM: The degree of systemic response after hepatic radiofrequency ablation (RFA) has not been well-compared to liver resection so far. This pilot study was designed to examine whether RFA, compared to liver resection, significantly varies concerning dissemination of circulating tumor cells and induction of different pro-inflammatory markers and liver-specific growth factors. PATIENTS AND METHODS: Patients with colorectal liver metastases were treated with RFA, a combination of RFA and resection or liver resection only. Blood samples of 18 patients were obtained at different time points and interleukin (IL)-6, hepatocyte growth factor (HGF) and 70-kD heat shock protein (HSP70) serum levels were determined by ELISA. Circulating tumor cells were detected with reverse transcription-polymerase chain reaction (RT-PCR) amplification of cytokeratin 20 (CK20) mRNA (CK20 RT-PCR). RESULTS: The detection of circulating tumor cells was not significantly different, but in two patients RFA induced tumor cell dissemination. Serum levels of IL-6 were strongly elevated after the operation without any significant differences between the treatment groups. The HGF ratio was significantly higher after RFA+resection compared to resection-alone and the HSP70 ratio also showed significantly higher values after RFA compared to resection alone. High postoperative IL-6 and HGF levels negatively influenced overall survival (OS) independently of the treatment group. CONCLUSION: This pilot study demonstrates that RFA might influence tumor cell dissemination. There exist detectable differences in serum factors between RFA and liver resection after the operation but this did not influence the overall survival of the patients. For all patients, high postoperative IL-6 and HGF levels are negative prognostic markers. Copyright
BACKGROUND/AIM: The degree of systemic response after hepatic radiofrequency ablation (RFA) has not been well-compared to liver resection so far. This pilot study was designed to examine whether RFA, compared to liver resection, significantly varies concerning dissemination of circulating tumor cells and induction of different pro-inflammatory markers and liver-specific growth factors. PATIENTS AND METHODS: Patients with colorectal liver metastases were treated with RFA, a combination of RFA and resection or liver resection only. Blood samples of 18 patients were obtained at different time points and interleukin (IL)-6, hepatocyte growth factor (HGF) and 70-kD heat shock protein (HSP70) serum levels were determined by ELISA. Circulating tumor cells were detected with reverse transcription-polymerase chain reaction (RT-PCR) amplification of cytokeratin 20 (CK20) mRNA (CK20 RT-PCR). RESULTS: The detection of circulating tumor cells was not significantly different, but in two patients RFA induced tumor cell dissemination. Serum levels of IL-6 were strongly elevated after the operation without any significant differences between the treatment groups. The HGF ratio was significantly higher after RFA+resection compared to resection-alone and the HSP70 ratio also showed significantly higher values after RFA compared to resection alone. High postoperative IL-6 and HGF levels negatively influenced overall survival (OS) independently of the treatment group. CONCLUSION: This pilot study demonstrates that RFA might influence tumor cell dissemination. There exist detectable differences in serum factors between RFA and liver resection after the operation but this did not influence the overall survival of the patients. For all patients, high postoperative IL-6 and HGF levels are negative prognostic markers. Copyright
Authors: Erik Velez; S Nahum Goldberg; Gaurav Kumar; Yuanguo Wang; Svetlana Gourevitch; Jacob Sosna; Tyler Moon; Christopher L Brace; Muneeb Ahmed Journal: Radiology Date: 2016-07-13 Impact factor: 11.105
Authors: Joseph P Erinjeri; Gabriel C Fine; Gosse J Adema; Muneeb Ahmed; Julius Chapiro; Martijn den Brok; Rafael Duran; Stephen J Hunt; D Thor Johnson; Jens Ricke; Daniel Y Sze; Beau Bosko Toskich; Bradford J Wood; David Woodrum; S Nahum Goldberg Journal: Radiology Date: 2019-04-23 Impact factor: 11.105
Authors: J Scott Goodwin; Leo L Tsai; David Mwin; Patricia Coutinho de Souza; Svayam Dialani; John T Moon; Zheng Zhang; Aaron K Grant; Muneeb Ahmed Journal: Magn Reson Imaging Date: 2021-04-24 Impact factor: 3.130
Authors: Yang Li; Na Huang; Chunlin Wang; Huanrong Ma; Minyu Zhou; Li Lin; Zhenhua Huang; Li Sun; Min Shi; Wangjun Liao Journal: Oncol Lett Date: 2018-06-25 Impact factor: 2.967
Authors: Sebastian Hinz; Christian Röder; Jürgen Tepel; Alexander Hendricks; Clemens Schafmayer; Thomas Becker; Holger Kalthoff Journal: BMC Cancer Date: 2015-12-16 Impact factor: 4.430