| Literature DB >> 25962819 |
Zhenzhen Gao1, Kuanhui Liu1, Weijun Tian1, Hongchao Wang1, Zhenguang Liu1, Youying Li1, Entao Li1, Cui Liu1, Xiuping Li1, Ranran Hou1, Chanjuan Yue1, Deyun Wang1, Yuanliang Hu2.
Abstract
The effects of two selenizing polysaccharides (sCAP2 and sGPS6) on immune function of murine peritoneal macrophages taking two non-selenizing polysaccharides (CAP and GPS) and modifier Na2SeO3 as control. In vitro test, the changes of selenizing polysaccharides, non-selenizing polysaccharides and Na2SeO3 on murine macrophages function were evaluated by phagocytosis and nitric oxide (NO) secretion tests. In vivo test, the mice were injected respectively with 0.2, 0.4 and 0.6 mg of sCAP2, sGPS6, CAP and GPS, or Na2SeO3 80 μg or normal saline 0.4 mL. The peritoneal macrophages were collected and cultured to determine the contents of TNF-α, IL-6 and IL-10 in supernatants by enzyme-linked immunosorbent assay. The results showed that sCAP2 and sGPS6 could significantly promote the phagocytosis and secretion of NO and three cytokines of macrophages in comparison with CAP and GPS. sCAP2 possessed the strongest activity. This indicates that selenylation modification can further improve the immune-enhancing activity of polysaccharide, and sCAP2 could be as a new immunopotentiator.Entities:
Keywords: Cytokines; Nitric oxide; Peritoneal macrophages; Phagocytosis; Selenizing Chinese angelica polysaccharides; Selenizing garlic polysaccharide
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Year: 2015 PMID： 25962819 DOI： 10.1016/j.intimp.2015.04.052
Source DB: PubMed Journal: Int Immunopharmacol ISSN： 1567-5769 Impact factor: 4.932