Jiangang Hou1, Masayuki Fujino2, Songjie Cai3, Qiang Ding4, Xiao-Kang Li3. 1. Department of Urology, Huashan Hospital, Fudan University, Shanghai, China. ; Division of Transplantation Immunology, National Research Institute for Child Health and Development, Tokyo, Japan. 2. Division of Transplantation Immunology, National Research Institute for Child Health and Development, Tokyo, Japan. ; AIDS Research Center, National Institute of Infectious Diseases, Tokyo, Japan. 3. Division of Transplantation Immunology, National Research Institute for Child Health and Development, Tokyo, Japan. 4. Department of Urology, Huashan Hospital, Fudan University, Shanghai, China.
Abstract
PURPOSE: Acute renal graft rejection can only be definitively diagnosed by renal biopsy. However, biopsies carry a risk of renal transplant injury and loss. Micro-CT is widely used in preclinical studies of small animals. Here, we propose micro-CT could noninvasively monitor and evaluate renal location and function in a mouse kidney transplant model. METHODS: Orthotopic kidney transplantation was performed in a BALB/c -to- C57BL/6j or C57BL/6j-to- C57BL/6j mouse model. After optimizing imaging techniques, five mice were imaged with micro-CT and the findings were verified histologically. RESULTS: Micro-CT can monitor and evaluate renal location and function after orthotopic kidney transplantation. There were no mice deaths while renal transplants were failure. CONCLUSION: We propose that graft micro-CT imaging is a new option that is noninvasive and specific, and can aid in early detection and follow-up of acute renal rejection. This method is potentially useful to improve posttransplant rejection monitoring.
PURPOSE: Acute renal graft rejection can only be definitively diagnosed by renal biopsy. However, biopsies carry a risk of renal transplant injury and loss. Micro-CT is widely used in preclinical studies of small animals. Here, we propose micro-CT could noninvasively monitor and evaluate renal location and function in a mouse kidney transplant model. METHODS: Orthotopic kidney transplantation was performed in a BALB/c -to- C57BL/6j or C57BL/6j-to- C57BL/6j mouse model. After optimizing imaging techniques, five mice were imaged with micro-CT and the findings were verified histologically. RESULTS: Micro-CT can monitor and evaluate renal location and function after orthotopic kidney transplantation. There were no mice deaths while renal transplants were failure. CONCLUSION: We propose that graft micro-CT imaging is a new option that is noninvasive and specific, and can aid in early detection and follow-up of acute renal rejection. This method is potentially useful to improve posttransplant rejection monitoring.
Authors: Stefan Reuter; Uta Schnöckel; Rita Schröter; Otmar Schober; Hermann Pavenstädt; Michael Schäfers; Gert Gabriëls; Eberhard Schlatter Journal: PLoS One Date: 2009-04-24 Impact factor: 3.240