José Caamaño1, Evangelina Musante1, Margarita Contreras2, Hernán Ulloa2, Carolina Reyes2, Verónica Inaipil2, Nicolás Saavedra3, Neftalí Guzmán4. 1. Banco de Sangre Hospital Dr. Hernán Henríquez Aravena, Temuco, Chile ; Facultad de Salud Universidad Santo Tomás, Temuco, Chile. 2. Banco de Sangre Hospital Dr. Hernán Henríquez Aravena, Temuco, Chile. 3. Centro de Biología Molecular y Farmacogenética, Núcleo Científico-Tecnológico en Biorecursos, Universidad de La Frontera, Temuco, Chile. 4. Escuela de Ciencias de la Salud, Universidad Católica de Temuco, Temuco, Chile ; Facultad de Ciencias de la Salud, Universidad San Sebastián, Concepción, Chile.
Abstract
BACKGROUND: Alloimmunization is an adverse effect of blood transfusions. In Chile, alloimmunization frequency is not established, and for this reason the aim of this study was to investigate the prevalence and specificity of red blood cell (RBC) alloantibodies in Chilean transfused subjects. METHODS: Records from 4,716 multi-transfused patients were analyzed. In these patients, antibody screening was carried out prior to cross-matching with a commercially available two-cell panel by the microcolum gel test, and samples with a positive screen were analyzed for the specificity of the alloantibody with a 16-cell identification panel. RESULTS: The incidence of RBC alloimmunization in transfused patients was 1.02% (48/4,716) with a higher prevalence in women (40/48). We detected 52 antibodies, the most frequent specificities identified were anti-E (30.8%), anti-K (26.9%), anti-D (7.7%), and anti-Fy(a) (5.8%). The highest incidence of alloantibodies was observed in cancer and gastroenterology patients. CONCLUSION: The data demonstrated a low alloimmunization frequency in Chilean transfused patients, principally associated with antibodies anti-E, anti-K, anti-D, and anti-Fy(a).
BACKGROUND: Alloimmunization is an adverse effect of blood transfusions. In Chile, alloimmunization frequency is not established, and for this reason the aim of this study was to investigate the prevalence and specificity of red blood cell (RBC) alloantibodies in Chilean transfused subjects. METHODS: Records from 4,716 multi-transfused patients were analyzed. In these patients, antibody screening was carried out prior to cross-matching with a commercially available two-cell panel by the microcolum gel test, and samples with a positive screen were analyzed for the specificity of the alloantibody with a 16-cell identification panel. RESULTS: The incidence of RBC alloimmunization in transfused patients was 1.02% (48/4,716) with a higher prevalence in women (40/48). We detected 52 antibodies, the most frequent specificities identified were anti-E (30.8%), anti-K (26.9%), anti-D (7.7%), and anti-Fy(a) (5.8%). The highest incidence of alloantibodies was observed in cancer and gastroenterology patients. CONCLUSION: The data demonstrated a low alloimmunization frequency in Chilean transfused patients, principally associated with antibodies anti-E, anti-K, anti-D, and anti-Fy(a).
Entities:
Keywords:
Alloimmunization; Red blood cell transfusion; Unexpected antibodies
Authors: Paola Rocco; Carmen Morales; Mauricio Moraga; Juan Francisco Miquel; Flavio Nervi; Elena Llop; Pilar Carvallo; Francisco Rothhammer Journal: Rev Med Chil Date: 2002-02 Impact factor: 0.553
Authors: Katie L Lannan; Julie Sahler; Sherry L Spinelli; Richard P Phipps; Neil Blumberg Journal: Blood Cells Mol Dis Date: 2012-09-13 Impact factor: 3.039