Montserrat Torres1, Tarik Gheit1, Sandrine McKay-Chopin1, Carmen Rodríguez2, Jorge Del Romero2, Raffaele Filotico3, Maria Gabriella Doná4, Marta Ortiz5, Massimo Tommasino6. 1. Infections and Cancer Biology Group, International Agency for Research on Cancer, 69008 Lyon, France. 2. Sandoval Health Centre, ES-28029 Madrid, Spain. 3. Unità Operativa complessa di Dermatologia Presidio Ospedaliero. A. Perrino, Brindisi, Italy. 4. STI/HIV Unit, San Gallicano Dermatologic Institute, IRCCS, Rome, Italy. 5. Retrovirus and Papillomavirus Unit, National Centre of Microbiology, Institute of Health Carlos III, Madrid, Spain. 6. Infections and Cancer Biology Group, International Agency for Research on Cancer, 69008 Lyon, France. Electronic address: tommasino@iarc.fr.
Abstract
BACKGROUND: Mucosal high-risk human papillomavirus (HPV) types benefit differently from the immunocompromised status of the host. So far it is not known whether a similar scenario holds for the large group of the β and γ cutaneous HPV types that appear to be present at several anatomical sites. METHODS: The presence of β (n=43) and γ (n=30) HPVs in the anal samples of 66 HIV-positive and 153 HIV-negative anonymized men who have sex with men (MSM) was determined by multiplex PCR, using type-specific primers and bead-based hybridization (Luminex technology). RESULTS: The prevalence of β and γ HPV infection was 65.6% and 68.2%, respectively, among HIV-positive MSM and 59.1% and 57.7%, respectively, among HIV-negative MSM. β-2 and γ-10 were found to be the most prevalent species in both groups. The prevalence of infection with HPV types of the species β-1 (P=0.02), β-3 (P=0.002), γ-6 (P=0.002), and γ-7 (P=0.02) was higher in HIV-positive than HIV-negative men. In contrast, the β-2 species was equally distributed in the two groups, while the γ-10 species was slightly affected by HIV status. CONCLUSIONS: These findings provide evidence that impairment of the host's immune surveillance impacts β and γ HPV infections differently.
BACKGROUND: Mucosal high-risk human papillomavirus (HPV) types benefit differently from the immunocompromised status of the host. So far it is not known whether a similar scenario holds for the large group of the β and γ cutaneous HPV types that appear to be present at several anatomical sites. METHODS: The presence of β (n=43) and γ (n=30) HPVs in the anal samples of 66 HIV-positive and 153 HIV-negative anonymized men who have sex with men (MSM) was determined by multiplex PCR, using type-specific primers and bead-based hybridization (Luminex technology). RESULTS: The prevalence of β and γ HPVinfection was 65.6% and 68.2%, respectively, among HIV-positive MSM and 59.1% and 57.7%, respectively, among HIV-negative MSM. β-2 and γ-10 were found to be the most prevalent species in both groups. The prevalence of infection with HPV types of the species β-1 (P=0.02), β-3 (P=0.002), γ-6 (P=0.002), and γ-7 (P=0.02) was higher in HIV-positive than HIV-negative men. In contrast, the β-2 species was equally distributed in the two groups, while the γ-10 species was slightly affected by HIV status. CONCLUSIONS: These findings provide evidence that impairment of the host's immune surveillance impacts β and γ HPV infections differently.
Authors: Emily M Nunes; Rossana V M López; Staci L Sudenga; Tarik Gheit; Massimo Tommasino; Maria L Baggio; Silvaneide Ferreira; Lenice Galan; Roberto C Silva; Eduardo Lazcano-Ponce; Anna R Giuliano; Luisa L Villa; Laura Sichero Journal: Virology Date: 2017-07-11 Impact factor: 3.616
Authors: Nancy M Cladel; Lynn R Budgeon; Timothy K Cooper; Karla K Balogh; Neil D Christensen; Roland Myers; Vladimir Majerciak; Deanna Gotte; Zhi-Ming Zheng; Jiafen Hu Journal: J Gen Virol Date: 2017-10 Impact factor: 3.891
Authors: Rachel L Winer; Tarik Gheit; Stephen Cherne; John Lin; Joshua E Stern; Mario Poljak; Qinghua Feng; Massimo Tommasino Journal: Papillomavirus Res Date: 2017-11-10