Literature DB >> 25957965

Soluble Glycoprotein Is Not Required for Ebola Virus Virulence in Guinea Pigs.

Thomas Hoenen1, Andrea Marzi1, Dana P Scott2, Friederike Feldmann2, Julie Callison1, David Safronetz1, Hideki Ebihara1, Heinz Feldmann1.   

Abstract

Ebola virus (EBOV) uses transcriptional editing to express several glycoproteins (GPs), including secreted soluble GP (sGP) and structural GP1,2, from a single gene. Recombinant viruses predominantly expressing GP1,2 are known to rapidly mutate and acquire an editing site predominantly expressing sGP in vivo, suggesting an important role of this protein during infection. Therefore, we generated a recombinant virus that is no longer able to express sGP and assessed its virulence in the EBOV guinea pig model. Surprisingly, although this virus remained genetically stable, it did not show any significant attenuation in vivo, showing that sGP is not required for virulence in this model. Published by Oxford University Press on behalf of the Infectious Diseases Society of America 2015. This work is written by (a) US Government employee(s) and is in the public domain in the US.

Entities:  

Keywords:  ebola virus; guinea pigs; mRNA editing; recombinant virus; reverse genetics; sGP; soluble glycoprotein; virulence

Mesh：

Substances：

Year:  2015        PMID： 25957965      PMCID： PMC4564536          DOI： 10.1093/infdis/jiv111

Source DB:  PubMed          Journal:  J Infect Dis        ISSN： 0022-1899            Impact factor:   5.226


  17 in total

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10.  The virion glycoproteins of Ebola viruses are encoded in two reading frames and are expressed through transcriptional editing.

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5.  Ebola Virus Infections in Nonhuman Primates Are Temporally Influenced by Glycoprotein Poly-U Editing Site Populations in the Exposure Material.

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6.  Conserved differences in protein sequence determine the human pathogenicity of Ebolaviruses.

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