| Literature DB >> 25954932 |
Anastasia N Naumenko, Vladimir A Timoshevskiy, Nicholas A Kinney, Alina A Kokhanenko, Becky S deBruyn, Diane D Lovin, Vladimir N Stegniy, David W Severson, Igor V Sharakhov, Maria V Sharakhova.
Abstract
Entities:
Year: 2015 PMID: 25954932 PMCID: PMC4425355 DOI: 10.1371/journal.pone.0127565
Source DB: PubMed Journal: PLoS One ISSN: 1932-6203 Impact factor: 3.240
Fig 3A landmark-guided two-step physical mapping approach on Cx. quinquefasciatus chromosomes.
Chromosomes 1, 2, and 3 are indicated by numbers. Short and long chromosome arms are indicated by letters p and q, respectively. Chromosomes are subdivided into 19 divisions and 72 bands. Genomic supercontigs are indicated by the last 1 to 4 digits of their accession numbers. Genetic markers are shown in brackets.
Fig 4Chromosome idiograms with positions of supercontigs and genetic markers.
FISH of two BAC clones of interest was performed in the presence of 2 additional BAC clones, and 18S rDNA used as landmarks for the chromosome arm identification (A-C). Positions of molecular landmarks and 2 BAC clones of interest are indicated by arrows. Mitotic chromosomes at metaphase were used for the rapid assignment of the genomic supercontigs to the chromosome bands (D). Longer prophase (E) or polytene chromosomes (F) were further utilized for ordering the genomic supercontigs within the band.