Rajanikanth M1, Ravi Prakash A2, Sreenath G3, Sonia Bai Jk4, Shyam Ndvn5. 1. Reader, Department of Oral and Maxillofacial Pathology, G. Pulla Reddy Dental College and Hospital , India . 2. Professor and HOD, Department of Oral and Maxillofacial Pathology, G. Pulla Reddy Dental College and Hospital , Kurnool, India . 3. Senior Lecturer, Department of Oral and Maxillofacial Pathology, G. Pulla Reddy Dental College and Hospital , Kurnool, India . 4. Post Graduate Student, Department of Oral and Maxillofacial Pathology, G. Pulla Reddy Dental College and Hospital , Kurnool, India . 5. Professor, Department of Oral and Maxillofacial Pathology, Government Dental College- Hyderabad, India .
Abstract
BACKGROUND: Universally accepted fixative is 10% formalin which has been used for preserving the tissues and their architecture. In certain conditions, formalin might not be readily available for immediate fixation. We here by explore more economical, eco-friendly and easily available solutions that can be used as transit media/ transporting media for tissue specimens. MATERIALS AND METHODS: The study included commonly available solutions like Spirit, Saline, Betadine solution, Hydrogen peroxide (H2O2), Local anesthesia (L.A), Rose water, Coconut oil, Coconut water, Ice cold water, Honey and Milk while keeping formalin as control. The fresh tissue sample was cut into multiple bits and placed in different containers for a period of 8 hours before transferring to formalin solution. CONCLUSION: Transit fixatives are very important in certain situations where formalin is not readily available. These fixatives can be used to fix the tissues for a period of at least 8 hours without causing any damage or distortion before they are fixed in formalin solution.
BACKGROUND: Universally accepted fixative is 10% formalin which has been used for preserving the tissues and their architecture. In certain conditions, formalin might not be readily available for immediate fixation. We here by explore more economical, eco-friendly and easily available solutions that can be used as transit media/ transporting media for tissue specimens. MATERIALS AND METHODS: The study included commonly available solutions like Spirit, Saline, Betadine solution, Hydrogen peroxide (H2O2), Local anesthesia (L.A), Rose water, Coconut oil, Coconut water, Ice cold water, Honey and Milk while keeping formalin as control. The fresh tissue sample was cut into multiple bits and placed in different containers for a period of 8 hours before transferring to formalin solution. CONCLUSION: Transit fixatives are very important in certain situations where formalin is not readily available. These fixatives can be used to fix the tissues for a period of at least 8 hours without causing any damage or distortion before they are fixed in formalin solution.