Ashoka Mahapatra1, Nupur Padhi2, Dharitri Mahapatra3, Mamta Bhatt4, Debasish Sahoo5, Swetlina Jena6, Debabrata Dash6, Nirupama Chayani7. 1. Associate Professor, Department of Microbiology, AIIMS , Bhubaneswar S.C.B. Medical College, Cuttack, Odisha, India . 2. Undergraduate Student, S.C.B. Medical College , Cuttack, Odisha, India . 3. Assistant Professor, Department of Microbiology, S.C.B. Medical College , Cuttack, Odisha, India . 4. Senior Resident, Department of Microbiology, S.C.B. Medical College , Cuttack, Odisha, India . 5. Senior Resident, Department of Microbiology, SUM Hospital , Bhubaneswar, Odisha, India . 6. Post Graduate Student Student, Department of Microbiology, S.C.B. Medical College , Cuttack, Odisha, India . 7. Professor and Head, Department of Microbiology, S.C.B. Medical College , Cuttack, Odisha, India .
Abstract
CONTEXT: A biofilm is a layer of microorganisms contained in a matrix (slime layer), which forms on surfaces in contact with water. Their presence in drinking water pipe networks can be responsible for a wide range of water quality and operational problems. AIM: To identify the bacterial isolates, obtained from water pipelines of kitchens, to evaluate the water quality & to study the biofilm producing capacity of the bacterial isolates from various sources. SETTINGS AND DESIGN: A prospective study using water samples from aqua guard & pipelines to kitchens of S.C.B Medical College hostels. MATERIALS AND METHODS: Standard biochemical procedures for bacterial identification, multiple tube culture & MPN count to evaluate water quality & tissue culture plate (TCP) method for biofilm detection was followed. STATISTICAL ANALYSIS: STATA software version 9.2 from STATA Corporation, College station road, 90 Houston, Texas was used for statistical analysis. RESULTS: One hundred eighty seven isolates were obtained from 45 water samples cultured. The isolates were Acinetobacter spp. (44), Pseudomonas spp.(41), Klebsiella spp.(36) & others . Biofilm was detected in (37) 19.78 % of the isolates (95% CI 30.08% -43.92%) including Acinetobacter spp.-10, Klebsiella spp. - 9, Pseudomonas spp. - 9, & others, majority (34) of which were from kitchen pipelines. CONCLUSION: Water from pipeline sources was unsatisfactory for consumption as the MPN counts were > 10. Most of the biofilm producers were gram negative bacilli & Pseudomonas & Acinetobacter spp. were strong (4+) biofilm producers.
CONTEXT: A biofilm is a layer of microorganisms contained in a matrix (slime layer), which forms on surfaces in contact with water. Their presence in drinking water pipe networks can be responsible for a wide range of water quality and operational problems. AIM: To identify the bacterial isolates, obtained from water pipelines of kitchens, to evaluate the water quality & to study the biofilm producing capacity of the bacterial isolates from various sources. SETTINGS AND DESIGN: A prospective study using water samples from aqua guard & pipelines to kitchens of S.C.B Medical College hostels. MATERIALS AND METHODS: Standard biochemical procedures for bacterial identification, multiple tube culture & MPN count to evaluate water quality & tissue culture plate (TCP) method for biofilm detection was followed. STATISTICAL ANALYSIS: STATA software version 9.2 from STATA Corporation, College station road, 90 Houston, Texas was used for statistical analysis. RESULTS: One hundred eighty seven isolates were obtained from 45 water samples cultured. The isolates were Acinetobacter spp. (44), Pseudomonas spp.(41), Klebsiella spp.(36) & others . Biofilm was detected in (37) 19.78 % of the isolates (95% CI 30.08% -43.92%) including Acinetobacter spp.-10, Klebsiella spp. - 9, Pseudomonas spp. - 9, & others, majority (34) of which were from kitchen pipelines. CONCLUSION:Water from pipeline sources was unsatisfactory for consumption as the MPN counts were > 10. Most of the biofilm producers were gram negative bacilli & Pseudomonas & Acinetobacter spp. were strong (4+) biofilm producers.
Entities:
Keywords:
Bacterial aggregates; Health risk; Microorganisms; Planktonic
Authors: Ehsan Asghari; Annika Kiel; Bernhard Peter Kaltschmidt; Martin Wortmann; Nadine Schmidt; Bruno Hüsgen; Andreas Hütten; Cornelius Knabbe; Christian Kaltschmidt; Barbara Kaltschmidt Journal: Microorganisms Date: 2021-05-04