| Literature DB >> 25954012 |
Elizabeth S Egan1, Rays H Y Jiang2, Mischka A Moechtar3, Natasha S Barteneva4, Michael P Weekes5, Luis V Nobre5, Steven P Gygi6, Joao A Paulo6, Charles Frantzreb3, Yoshihiko Tani7, Junko Takahashi7, Seishi Watanabe8, Jonathan Goldberg3, Aditya S Paul3, Carlo Brugnara9, David E Root10, Roger C Wiegand10, John G Doench10, Manoj T Duraisingh11.
Abstract
Efforts to identify host determinants for malaria have been hindered by the absence of a nucleus in erythrocytes, which precludes genetic manipulation in the cell in which the parasite replicates. We used cultured red blood cells derived from hematopoietic stem cells to carry out a forward genetic screen for Plasmodium falciparum host determinants. We found that CD55 is an essential host factor for P. falciparum invasion. CD55-null erythrocytes were refractory to invasion by all isolates of P. falciparum because parasites failed to attach properly to the erythrocyte surface. Thus, CD55 is an attractive target for the development of malaria therapeutics. Hematopoietic stem cell-based forward genetic screens may be valuable for the identification of additional host determinants of malaria pathogenesis.Entities:
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Year: 2015 PMID： 25954012 PMCID： PMC4465434 DOI： 10.1126/science.aaa3526
Source DB: PubMed Journal: Science ISSN： 0036-8075 Impact factor: 47.728