Literature DB >> 2595370

Volatilization of high molecular weight DNA by pulsed laser ablation of frozen aqueous solutions.

R W Nelson1, M J Rainbow, D E Lohr, P Williams.   

Abstract

DNA has been volatilized by pulsed laser ablation of a thin film of a frozen aqueous DNA solution. The target film was irradiated in vacuum by a pulsed laser at power densities sufficient to ablate the ice matrix. The expanding ablated water vapor entrained embedded DNA molecules and expelled them into the gas phase. Ejection of DNA molecules as large as 410 kilodaltons was verified by collection of the ablation products and subsequent mass analysis by polyacrylamide gel electrophoresis with autoradiographic detection.

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Year:  1989        PMID： 2595370     DOI： 10.1126/science.2595370

Source DB:  PubMed          Journal:  Science        ISSN： 0036-8075            Impact factor:   47.728


  9 in total

1.  Infrared laser ablation sample transfer for MALDI and electrospray.

Authors:  Sung-Gun Park; Kermit King Murray
Journal:  J Am Soc Mass Spectrom       Date:  2011-05-28       Impact factor: 3.109

Review 2.  Mass spectrometric immunoassay revisited.

Authors:  Randall W Nelson; Chad R Borges
Journal:  J Am Soc Mass Spectrom       Date:  2011-03-08       Impact factor: 3.109

3.  Ice as a matrix for IR-matrix-assisted laser desorption/ionization: mass spectra from a protein single crystal.

Authors:  S Berkenkamp; M Karas; F Hillenkamp
Journal:  Proc Natl Acad Sci U S A       Date:  1996-07-09       Impact factor: 11.205

4.  Direct comparison of infrared and ultraviolet wavelength matrix-assisted laser desorption/ionization mass spectrometry of proteins.

Authors:  S Niu; W Zhang; B T Chait
Journal:  J Am Soc Mass Spectrom       Date:  1998-01       Impact factor: 3.109

5.  Infrared, surface-assisted laser desorption ionization mass spectrometry on frozen aqueous solutions of proteins and peptides using suspensions of organic solids.

Authors:  P Kraft; S Alimpiev; E Dratz; J Sunner
Journal:  J Am Soc Mass Spectrom       Date:  1998-09       Impact factor: 3.109

6.  Exploring infrared wavelength matrix-assisted laser desorption/ionization of proteins with delayed-extraction time-of-flight mass spectrometry.

Authors:  W Zhang; S Niu; B T Chait
Journal:  J Am Soc Mass Spectrom       Date:  1998-09       Impact factor: 3.109

7.  Mapping antiretroviral drugs in tissue by IR-MALDESI MSI coupled to the Q Exactive and comparison with LC-MS/MS SRM assay.

Authors:  Jeremy A Barry; Guillaume Robichaud; Mark T Bokhart; Corbin Thompson; Craig Sykes; Angela D M Kashuba; David C Muddiman
Journal:  J Am Soc Mass Spectrom       Date:  2014-04-18       Impact factor: 3.109

8.  Analysis of oligodeoxynucleotides by negative-ion matrix-assisted laser desorption mass spectrometry.

Authors:  G J Currie; J R Yates
Journal:  J Am Soc Mass Spectrom       Date:  1993-12       Impact factor: 3.109

9.  Substrate-Mediated Laser Ablation under Ambient Conditions for Spatially-Resolved Tissue Proteomics.

Authors:  Benoit Fatou; Maxence Wisztorski; Cristian Focsa; Michel Salzet; Michael Ziskind; Isabelle Fournier
Journal:  Sci Rep       Date:  2015-12-17       Impact factor: 4.379
  9 in total

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