Ping Xu1, Yan Li1, Shuyong Yang1, Haiyuan Yang1, Juan Tang1, Mingzhe Li2. 1. Department of Stomatology, Chengdu Military General Hospital, NO.270, Rongdu Road, Jingniu Zone, Chengdu, 610038, Sichuan, P. R. China. 2. Department of Stomatology, Chengdu Military General Hospital, NO.270, Rongdu Road, Jingniu Zone, Chengdu, 610038, Sichuan, P. R. China. Electronic address: biolimingzhe@163.com.
Abstract
OBJECTIVE: The aim of this study was to investigate the roles and mechanisms of Micro-ribonucleic acid 143 (miR-143) in oral squamous cell carcinoma cells. STUDY DESIGN: Following the detection of miR-143 expression, cell proliferation, migration, and invasion assays and Western blot analysis were conducted in the oral squamous cell carcinoma (OSCC) cell lines. RESULTS: We found that the expression of miR-143 was significantly decreased in the OSCC cell lines (SCC-4, Tca-8113, CAL-27) and tumor tissues. Meanwhile, miR-143 was significantly correlated with the migration and invasion in OSCC cell lines. Further investigation revealed that the expression level of miR-143 was opposite to that of its potential target gene-CD44 v3 and was also related to phospho-c-Met activation. CONCLUSIONS: miR-143 could exert significantly suppressive effects on the ability of migration and invasion in OSCC cell lines, and the mechanism of this might be related to the activity of phospho-c-met though the CD44 v3/HGF signal. miR-143 could thus provide new applications for the treatment of OSCC.
OBJECTIVE: The aim of this study was to investigate the roles and mechanisms of Micro-ribonucleic acid 143 (miR-143) in oral squamous cell carcinoma cells. STUDY DESIGN: Following the detection of miR-143 expression, cell proliferation, migration, and invasion assays and Western blot analysis were conducted in the oral squamous cell carcinoma (OSCC) cell lines. RESULTS: We found that the expression of miR-143 was significantly decreased in the OSCC cell lines (SCC-4, Tca-8113, CAL-27) and tumor tissues. Meanwhile, miR-143 was significantly correlated with the migration and invasion in OSCC cell lines. Further investigation revealed that the expression level of miR-143 was opposite to that of its potential target gene-CD44 v3 and was also related to phospho-c-Met activation. CONCLUSIONS:miR-143 could exert significantly suppressive effects on the ability of migration and invasion in OSCC cell lines, and the mechanism of this might be related to the activity of phospho-c-met though the CD44 v3/HGF signal. miR-143 could thus provide new applications for the treatment of OSCC.