Renata Falchete do Prado1, Fernanda Saraiva de Oliveira2, Rodrigo Dias Nascimento3, Luana Marotta Reis de Vasconcellos2, Yasmin Rodarte Carvalho2, Carlos Alberto Alves Cairo4. 1. Department of Bioscience and Oral Diagnosis, Institute of Science and Technology, UNESP - Univ Estadual Paulista, São José dos Campos (SP), School of Dentistry, Av. Engenheiro Francisco José Longo, 777, São José dos Campos 12245-000, SP, Brazil. Electronic address: renatafalchete@hotmail.com. 2. Department of Bioscience and Oral Diagnosis, Institute of Science and Technology, UNESP - Univ Estadual Paulista, São José dos Campos (SP), School of Dentistry, Av. Engenheiro Francisco José Longo, 777, São José dos Campos 12245-000, SP, Brazil. 3. Department of Diagnosis and Surgery Institute of Science and Technology, UNESP - Univ Estadual Paulista, São José dos Campos (SP), School of Dentistry, Av. Engenheiro Francisco José Longo, 777, São José dos Campos 12245-000, SP, Brazil. 4. Division of Materials, Air and Space Institute, CTA, Praça Mal. do Ar Eduardo Gomes, 14, São José dos Campos 12904-000, SP, Brazil.
Abstract
OBJECTIVE: This study analyzed the behavior of human osteoblasts cultured on porous titanium specimens, with and without biomimetic treatment, compared to dense titanium. DESIGN: The experiment had seven groups: Group 1: cells cultured on polystyrene of culture plate wells; Group 2: cells cultured on dense titanium specimen; Group 3: specimen with 33.79% of pores; Group 4: 41.79% of pores; Groups 5, 6 and 7: specimens similar to groups 2, 3 and 4, yet with biomimetic treatment. Real time-polymerase chain reaction with reverse transcription of the following genes was performed: prostaglandin E2 synthase, integrin β1, osterix, Runx2, Interleukin 6, macrophage colony stimulating factor, apolipoprotein E and others. The study achieved data on cell adhesion, growth and viability, total protein content, alkaline phosphatase activity and quantity of mineralized nodule formations. Data were statistically evaluated. RESULTS: Adherent cells and alkaline phosphatase activity were similar in titanium specimens, regardless of the groups. Biomimetic treatment reduced the total protein activity and the viability of tested cells. Most tested genes had statistically similar expression in all groups. CONCLUSION: The tested porosities did not cause alterations in osteoblast behavior and the biomimetic treatment impaired the biocompatibility of titanium causing cytotoxicity.
OBJECTIVE: This study analyzed the behavior of human osteoblasts cultured on porous titanium specimens, with and without biomimetic treatment, compared to dense titanium. DESIGN: The experiment had seven groups: Group 1: cells cultured on polystyrene of culture plate wells; Group 2: cells cultured on dense titanium specimen; Group 3: specimen with 33.79% of pores; Group 4: 41.79% of pores; Groups 5, 6 and 7: specimens similar to groups 2, 3 and 4, yet with biomimetic treatment. Real time-polymerase chain reaction with reverse transcription of the following genes was performed: prostaglandin E2 synthase, integrin β1, osterix, Runx2, Interleukin 6, macrophage colony stimulating factor, apolipoprotein E and others. The study achieved data on cell adhesion, growth and viability, total protein content, alkaline phosphatase activity and quantity of mineralized nodule formations. Data were statistically evaluated. RESULTS: Adherent cells and alkaline phosphatase activity were similar in titanium specimens, regardless of the groups. Biomimetic treatment reduced the total protein activity and the viability of tested cells. Most tested genes had statistically similar expression in all groups. CONCLUSION: The tested porosities did not cause alterations in osteoblast behavior and the biomimetic treatment impaired the biocompatibility of titanium causing cytotoxicity.
Authors: Tabata P Sato; Bruno V M Rodrigues; Daphne C R Mello; Eliseu A Münchow; Juliana S Ribeiro; João Paulo B Machado; Luana M R Vasconcellos; Anderson O Lobo; Marco C Bottino; Alexandre L S Borges Journal: Clin Oral Investig Date: 2020-10-13 Impact factor: 3.573
Authors: Natalia M Martinelli; Maria Julia G Ribeiro; Ritchelli Ricci; Miller A Marques; Anderson Oliveira Lobo; Fernanda Roberta Marciano Journal: Materials (Basel) Date: 2018-08-29 Impact factor: 3.623