Literature DB >> 25953352

A Toolkit of CRISPR-Based Genome Editing Systems in Drosophila.

Jiang Xu1, Xingjie Ren2, Jin Sun2, Xia Wang2, Huan-Huan Qiao2, Bo-Wen Xu2, Lu-Ping Liu3, Jian-Quan Ni4.   

Abstract

The last couple of years have witnessed an explosion in development of CRISPR-based genome editing technologies in cell lines as well as in model organisms. In this review, we focus on the applications of this popular system in Drosophila. We discuss the effectiveness of the CRISPR/Cas9 systems in terms of delivery, mutagenesis detection, parameters affecting efficiency, and off-target issues, with an emphasis on how to apply this powerful tool to characterize gene functions.
Copyright © 2015 Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, and Genetics Society of China. Published by Elsevier Ltd. All rights reserved.

Entities:  

Keywords:  CRISPR; Cas9; Drosophila; Genome editing; Off-target; sgRNA

Mesh：

Substances：

Year:  2015        PMID： 25953352     DOI： 10.1016/j.jgg.2015.02.007

Source DB:  PubMed          Journal:  J Genet Genomics        ISSN： 1673-8527            Impact factor:   4.275


  14 in total

1.  CRISPR-Based Transcriptional Activation in Drosophila.

Authors:  Yuting Han; Xinyi Lu; Yutong Li; Yuhao Qiu; Xizhi Dong; Xiaochen Li; Xu Si; Qingfei Liu; Jian-Quan Ni
Journal:  Methods Mol Biol       Date:  2022

Review 2.  Drosophila melanogaster: a simple genetic model of kidney structure, function and disease.

Authors:  Julian A T Dow; Matias Simons; Michael F Romero
Journal:  Nat Rev Nephrol       Date:  2022-04-11       Impact factor: 42.439

3.  High Efficiency CRISPR/Cas9-mediated Gene Editing in Primary Human T-cells Using Mutant Adenoviral E4orf6/E1b55k "Helper" Proteins.

Authors:  Kamila S Gwiazda; Alexandra E Grier; Jaya Sahni; Stephen M Burleigh; Unja Martin; Julia G Yang; Nicholas A Popp; Michelle C Krutein; Iram F Khan; Kyle Jacoby; Michael C Jensen; David J Rawlings; Andrew M Scharenberg
Journal:  Mol Ther       Date:  2016-05-16       Impact factor: 11.454

Review 4.  Progress and Prospects of CRISPR/Cas Systems in Insects and Other Arthropods.

Authors:  Dan Sun; Zhaojiang Guo; Yong Liu; Youjun Zhang
Journal:  Front Physiol       Date:  2017-09-06       Impact factor: 4.566

5.  Efficient Genome Editing of a Facultative Thermophile Using Mesophilic spCas9.

Authors:  Ioannis Mougiakos; Elleke F Bosma; Koen Weenink; Eric Vossen; Kirsten Goijvaerts; John van der Oost; Richard van Kranenburg
Journal:  ACS Synth Biol       Date:  2017-02-16       Impact factor: 5.110

6.  Efficient Genome Editing of Magnetospirillum magneticum AMB-1 by CRISPR-Cas9 System for Analyzing Magnetotactic Behavior.

Authors:  Haitao Chen; Sheng-Da Zhang; Linjie Chen; Yao Cai; Wei-Jia Zhang; Tao Song; Long-Fei Wu
Journal:  Front Microbiol       Date:  2018-07-17       Impact factor: 5.640

7.  A Genetic Mosaic Screen Reveals Ecdysone-Responsive Genes Regulating Drosophila Oogenesis.

Authors:  Elizabeth T Ables; Grace H Hwang; Danielle S Finger; Taylor D Hinnant; Daniela Drummond-Barbosa
Journal:  G3 (Bethesda)       Date:  2016-08-09       Impact factor: 3.154

Review 8.  Drosophila: An Emergent Model for Delineating Interactions between the Circadian Clock and Drugs of Abuse.

Authors:  Aliza K De Nobrega; Lisa C Lyons
Journal:  Neural Plast       Date:  2017-12-17       Impact factor: 3.599

9.  Characterizing a thermostable Cas9 for bacterial genome editing and silencing.

Authors:  Ioannis Mougiakos; Prarthana Mohanraju; Elleke F Bosma; Valentijn Vrouwe; Max Finger Bou; Mihris I S Naduthodi; Alex Gussak; Rudolf B L Brinkman; Richard van Kranenburg; John van der Oost
Journal:  Nat Commun       Date:  2017-11-21       Impact factor: 14.919

10.  A simple method for imaging axonal transport in aging neurons using the adult Drosophila wing.

Authors:  Alessio Vagnoni; Simon L Bullock
Journal:  Nat Protoc       Date:  2016-08-18       Impact factor: 13.491
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