Juan Ping1, Zhi-Hui Shen2, Bao-Quan Wang1, Na Zhao2, Rui Li3, Mian Li4, Xiao-Bin Pang4, Chuan-Bo Chen5. 1. Department of Gastroenterology, The Huaihe Clincal College of Henan University, Kaifeng 475004, Henan Province, China. 2. Department of Hematology and Oncology, The Tumour Hospital of Zhengzhou University, Zhengzhou 450009, Henan Province, China. 3. Department of Hematology and Oncology, The Xijing Hospital of Fourth Military Medical University, Xi'an 730011, Shanxi Province, China. 4. Department of Chemibiology, The Pharmaceutical College, Henan University, Kaifeng 475001, Henan Province, China. 5. Department of Gastroenterology, The Huaihe Clincal College of Henan University, Kaifeng 475004, Henan Province, China. E-mail: chenchuanbo@henan.edu.cn.
Abstract
OBJECTIVE: To explore the effects of aptamer-siRNA nucleic acid compound on growth and apoptosis in myeloid leukemia cell line K562. METHODS: the changes of cellular morphology and structure were observed by using fluorescence microscope, laser confocal microscope, JEM-4000EX transmission electron microscopy; MTT assay were performed to evaluate the sensibility of K562 cells to aptamer-siRNA compound, the apoptosis was detected by DNA gel electro-phoresis. RESULTS: The remarkably changes of morphology and structure of K562 cells treated with 200 µmol/L aptamer-siRNA were observed under fluorescence microscopy and electromicroscopy. As compared with control, the aptamer-siRNA compound showed more inhibitory effect on K562 cells and there was significant difference (P<0.05). The MTT assay showed that the IC50 value of aptamer-siRNA compound for K562 cells was 150 µmol/L. According to agarose gel electrophoresis observation, when the aptamer-siRNA compound showed effect on K562 cells, the typical DNA lader could be observed. CONCLUSION: The aptamer-siRNA compound can significantly induce K562 cell apoptosis, and provide reference for gene therapy of patients with chronic myelocytic lenkemia.
OBJECTIVE: To explore the effects of aptamer-siRNA nucleic acid compound on growth and apoptosis in myeloid leukemia cell line K562. METHODS: the changes of cellular morphology and structure were observed by using fluorescence microscope, laser confocal microscope, JEM-4000EX transmission electron microscopy; MTT assay were performed to evaluate the sensibility of K562 cells to aptamer-siRNA compound, the apoptosis was detected by DNA gel electro-phoresis. RESULTS: The remarkably changes of morphology and structure of K562 cells treated with 200 µmol/L aptamer-siRNA were observed under fluorescence microscopy and electromicroscopy. As compared with control, the aptamer-siRNA compound showed more inhibitory effect on K562 cells and there was significant difference (P<0.05). The MTT assay showed that the IC50 value of aptamer-siRNA compound for K562 cells was 150 µmol/L. According to agarose gel electrophoresis observation, when the aptamer-siRNA compound showed effect on K562 cells, the typical DNA lader could be observed. CONCLUSION: The aptamer-siRNA compound can significantly induce K562 cell apoptosis, and provide reference for gene therapy of patients with chronic myelocytic lenkemia.