Madeline A Fujishiro 1 , Andrea V Scorza 2 , Jody L Gookin 3 , Michael R Lappin 2 Show Affiliations »
Abstract
OBJECTIVES: Coxiella burnetii is an obligate intracellular bacterium that is found worldwide, is associated or suggested to be associated with reproductive abnormalities in a number of species including cats, and is the cause of Q fever in humans. In a previous study, C burnetii DNA was amplified from the uterine tissues of 8.5% of client-owned cats in the USA but reproductive history was unknown and histopathological examination was not performed. In this study, uterine tissues of 26 normal cats and 11 cats with histopathological evidence of uterine disease or other reproductive abnormalities were evaluated for the presence of C burnetii. METHODS: A PCR assay that amplifies the repetitive transposon-like region (Trans 1 and 2) and a PCR assay that amplifies the IS-1111-insertion sequence (IS-1111) were optimised and applied to the DNA extracts. The sensitivity threshold of both PCR assays was 12 pg/µl. Positive samples were evaluated for the presence of the organism using immunohistochemistry performed on paraffin-embedded tissue. RESULTS: Amplicons of the expected size developed in three samples (one from a cat with reproductive abnormalities) in the IS-1111 assay; however, there was not enough DNA for sequence analysis. Immunohistochemical analysis was used to further evaluate these three samples and was negative for C burnetii. While C burnetii could not be confirmed by sequence analysis or immunohistochemistry, the PCR positive prevalence rate (8.1%) was similar to that published previously. CONCLUSIONS AND RELEVANCE: Biosafety precautions should be taken when working with cats that are aborting or parturient. Further research should be performed to evaluate the role that C burnetii may play in reproductive abnormalities in cats. © ISFM and AAFP 2015.
OBJECTIVES: Coxiella burnetii is an obligate intracellular bacterium that is found worldwide, is associated or suggested to be associated with reproductive abnormalities in a number of species including cats , and is the cause of Q fever in humans . In a previous study, C burnetii DNA was amplified from the uterine tissues of 8.5% of client-owned cats in the USA but reproductive history was unknown and histopathological examination was not performed. In this study, uterine tissues of 26 normal cats and 11 cats with histopathological evidence of uterine disease or other reproductive abnormalities were evaluated for the presence of C burnetii. METHODS: A PCR assay that amplifies the repetitive transposon-like region (Trans 1 and 2) and a PCR assay that amplifies the IS-1111-insertion sequence (IS-1111) were optimised and applied to the DNA extracts. The sensitivity threshold of both PCR assays was 12 pg/µl. Positive samples were evaluated for the presence of the organism using immunohistochemistry performed on paraffin -embedded tissue. RESULTS: Amplicons of the expected size developed in three samples (one from a cat with reproductive abnormalities) in the IS-1111 assay; however, there was not enough DNA for sequence analysis. Immunohistochemical analysis was used to further evaluate these three samples and was negative for C burnetii. While C burnetii could not be confirmed by sequence analysis or immunohistochemistry, the PCR positive prevalence rate (8.1%) was similar to that published previously. CONCLUSIONS AND RELEVANCE: Biosafety precautions should be taken when working with cats that are aborting or parturient. Further research should be performed to evaluate the role that C burnetii may play in reproductive abnormalities in cats . © ISFM and AAFP 2015.
Entities: Chemical
Disease
Species
Mesh： See more »
Year: 2015
PMID： 25944579 DOI： 10.1177/1098612X15584693
Source DB: PubMed Journal: J Feline Med Surg ISSN： 1098-612X Impact factor: 2.015