Literature DB >> 259425

Inhibition of deacylation and improvement in N-hydroxysuccinimide ester modification of phenylalanyl-tRNA by dimethyl sulfoxide.

M A Reuben, I J Kusnezov, E Wickstrom.   

Abstract

We have found that dimethyl sulfoxide significantly inhibits deacylation of Phe-tRNA. This allows a high pH in N-hydroxysuccinimide ester reactions while maintaining a high level of aminoacylated tRNA, improving the overall yield of the Phe-tRNA modification reaction.

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Year:  1979        PMID： 259425     DOI： 10.1016/0005-2787(79)90099-6

Source DB:  PubMed          Journal:  Biochim Biophys Acta        ISSN： 0006-3002


  3 in total

1.  tRNA dynamics on the ribosome during translation.

Authors:  Scott C Blanchard; Harold D Kim; Ruben L Gonzalez; Joseph D Puglisi; Steven Chu
Journal:  Proc Natl Acad Sci U S A       Date:  2004-08-18       Impact factor: 11.205

2.  Crosslinking of Escherichia coli 50S ribosomal subunits with chlorambucilyl oligoprolyl phenylalanyl-tRNA molecular rulers.

Authors:  K K Parker; E Wickstrom
Journal:  Nucleic Acids Res       Date:  1983-01-25       Impact factor: 16.971

3.  Molecular rulers for measuring RNA structure: sites of crosslinking in chlorambucilyl-phenylalanyl-tRNAPhe (yeast) and chlorambucilyl-pentadecaprolyl-phenylalanyl-tRNAPhe (yeast) intramolecularly crosslinked in aqueous solution.

Authors:  E Wickstrom; L S Behlen; M A Reuben; P R Ainpour
Journal:  Proc Natl Acad Sci U S A       Date:  1981-04       Impact factor: 11.205
  3 in total

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