Literature DB >> 25940929

MALDI-TOF mass spectrometry for early identification of bacteria grown in blood culture bottles.

Jean-Benoît Zabbe1, Laura Zanardo1, Francis Mégraud1, Emilie Bessède1.   

Abstract

This note reports an interesting way to rapidly identify bacteria grown from blood culture bottles. Chocolate agar plates were inoculated with 1 drop of the positive blood bottle medium. After a 3-hour incubation, the growth veil was submitted to MALDI-TOF mass spectrometry: 77% of the bacteria present have been correctly identified.
Copyright © 2015 Elsevier B.V. All rights reserved.

Entities:  

Keywords:  Bacteremia diagnosis; Rapid direct identification; Sepsityper alternative; Septic patients

Mesh:

Substances:

Year:  2015        PMID: 25940929     DOI: 10.1016/j.mimet.2015.04.009

Source DB:  PubMed          Journal:  J Microbiol Methods        ISSN: 0167-7012            Impact factor:   2.363


  11 in total

1.  Direct Identification of 80 Percent of Bacteria from Blood Culture Bottles by Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry Using a 10-Minute Extraction Protocol.

Authors:  Loïc Simon; Estelle Ughetto; Alice Gaudart; Nicolas Degand; Romain Lotte; Raymond Ruimy
Journal:  J Clin Microbiol       Date:  2019-01-30       Impact factor: 5.948

2.  How small modifications in laboratory workflow of blood cultures can have a significant impact on time to results.

Authors:  B Van den Poel; A Klak; S Desmet; J Verhaegen
Journal:  Eur J Clin Microbiol Infect Dis       Date:  2018-06-30       Impact factor: 3.267

3.  Improved blood culture workflow for faster identification of KPC-producing Enterobacterales.

Authors:  Bruna Mara Silva Seco; Juliana Coutinho Campos; Darlan Augusto da Costa Rocha; Aline Valerio de Lima; Fernanda Filomena de Oliveira; Mara Elisa Borsato Lemo; Suely Carlos Ferreira Sampaio; Jorge Luiz Mello Sampaio
Journal:  Braz J Microbiol       Date:  2018-12-06       Impact factor: 2.476

4.  Performance and potential clinical impact of Alfred60AST (Alifax®) for direct antimicrobial susceptibility testing on positive blood culture bottles.

Authors:  Bea Van den Poel; Philippe Meersseman; Yves Debaveye; Adrian Klak; Jan Verhaegen; Stefanie Desmet
Journal:  Eur J Clin Microbiol Infect Dis       Date:  2019-10-17       Impact factor: 3.267

5.  MALDI-ToF short incubation identification from blood cultures is associated with reduced length of hospitalization and a decrease in bacteremia associated mortality.

Authors:  J A Delport; A Strikwerda; A Armstrong; D Schaus; M John
Journal:  Eur J Clin Microbiol Infect Dis       Date:  2017-01-20       Impact factor: 3.267

6.  Real-world Time to Positivity of 2 Widely Used Commercial Blood Culture Systems in Patients With Severe Manifestations of Sepsis: An Analysis of the FABLED Study.

Authors:  Guillaume Butler-Laporte; Cedric P Yansouni; Katryn Paquette; Alexander Lawandi; Sarah N Stabler; Murtaza Akhter; Adam C Davidson; Marko Gavric; Rehman Jinah; Zahid Saeed; Koray Demir; Sassan Sangsari; Kelly Huang; Amirali Mahpour; Chris Shamatutu; Chelsea Caya; Jean-Marc Troquet; Greg Clark; Titus Wong; Todd C Lee; Robert Stenstrom; David Sweet; Matthew P Cheng
Journal:  Open Forum Infect Dis       Date:  2020-08-23       Impact factor: 3.835

7.  Direct blood culturing on solid medium outperforms an automated continuously monitored broth-based blood culture system in terms of time to identification and susceptibility testing.

Authors:  E A Idelevich; B Grünastel; G Peters; K Becker
Journal:  New Microbes New Infect       Date:  2015-12-23

8.  Same-Day Identification and Antimicrobial Susceptibility Testing of Bacteria in Positive Blood Culture Broths Using Short-Term Incubation on Solid Medium with the MicroFlex LT, Vitek-MS, and Vitek2 Systems.

Authors:  Jihye Ha; Sung Kuk Hong; Geum Hee Han; Myungsook Kim; Dongeun Yong; Kyungwon Lee
Journal:  Ann Lab Med       Date:  2018-05       Impact factor: 3.464

9.  Rapid identification of respiratory bacterial pathogens from bronchoalveolar lavage fluid in cattle by MALDI-TOF MS.

Authors:  Laura Van Driessche; Jade Bokma; Piet Deprez; Freddy Haesebrouck; Filip Boyen; Bart Pardon
Journal:  Sci Rep       Date:  2019-12-05       Impact factor: 4.379

10.  Testing the Applicability of MALDI-TOF MS as an Alternative Stock Identification Method in a Cryptic Species Complex.

Authors:  Gabor Maasz; Zita Zrínyi; Istvan Fodor; Nóra Boross; Zoltán Vitál; Dóra Ildikó Kánainé Sipos; Balázs Kovács; Szilvia Melegh; Péter Takács
Journal:  Molecules       Date:  2020-07-14       Impact factor: 4.411

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