| Literature DB >> 25940676 |
Shweta Fulsundar1, Heramb M Kulkarni2, Medicharla V Jagannadham2, Rashmi Nair1, Sravani Keerthi2, Pooja Sant1, Karishma Pardesi3, Jayesh Bellare4, Balu Ananda Chopade5.
Abstract
Acinetobacter radioresistens is an important member of genus Acinetobacter from a clinical point of view. In the present study, we report that a clinical isolate of A. radioresistens releases outer membrane vesicles (OMVs) under in vitro growth conditions. OMVs were released in distinctive size ranges with diameters from 10 to 150 nm as measured by the dynamic light scattering (DLS) technique. Additionally, proteins associated with or present into OMVs were identified using LC-ESI-MS/MS. A total of 71 proteins derived from cytosolic, cell membrane, periplasmic space, outer membrane (OM), extracellular and undetermined locations were found in OMVs. The initial characterization of the OMV proteome revealed a correlation of some proteins to biofilm, quorum sensing, oxidative stress tolerance, and cytotoxicity functions. Thus, the OMVs of A. radioresistens are suggested to play a role in biofilm augmentation and virulence possibly by inducing apoptosis.Entities:
Keywords: Acinetobacter radioresistens; Apoptosis; Biofilm augmentation; Cytotoxicity; OMV
Mesh：
Substances：
Year: 2015 PMID： 25940676 DOI： 10.1016/j.micpath.2015.04.005
Source DB: PubMed Journal: Microb Pathog ISSN： 0882-4010 Impact factor: 3.738