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Literature DB >> 25935417

Differential regulation of Connexin50 and Connexin46 by PI3K signaling.

Jennifer M Martinez¹, Hong-Zhan Wang¹, Richard Z Lin¹, Peter R Brink¹, Thomas W White².

Abstract

Gap junction channels can modify their activity in response to cell signaling pathways. Here, we demonstrate that Connexin50 (Cx50) coupling, but not Connexin46 (Cx46), increased when co-expressed with a constitutively active p110α subunit of PI3K in Xenopus oocytes. In addition, inhibition of PI3K signaling by blocking p110α, or Akt, significantly decreased gap junctional conductance in Cx50 transfected HeLa cells, with no effect on Cx46. Alterations in coupling levels were not a result of reduced Cx50 unitary conductance, suggesting that changes in the number of active channels were responsible. These data indicate that Cx50 is specifically regulated by the PI3K signaling pathway.

Entities: CellLine Chemical Disease Gene Mutation Species

Keywords: Akt; Connexin; Gap junction; Lens; PI3K

Mesh：

Substances：

Year: 2015 PMID： 25935417 PMCID： PMC4433579 DOI： 10.1016/j.febslet.2015.04.029

Source DB: PubMed Journal: FEBS Lett ISSN： 0014-5793 Impact factor: 4.124

44 in total

1. Expression of gap junctional proteins in Xenopus oocyte pairs.

Authors: L Ebihara
Journal: Methods Enzymol Date: 1992 Impact factor: 1.600

2. A Calpha model for the transmembrane alpha helices of gap junction intercellular channels.

Authors: Sarel J Fleishman; Vinzenz M Unger; Mark Yeager; Nir Ben-Tal
Journal: Mol Cell Date: 2004-09-24 Impact factor: 17.970

Review 3. Lens gap junctions in growth, differentiation, and homeostasis.

Authors: Richard T Mathias; Thomas W White; Xiaohua Gong
Journal: Physiol Rev Date: 2010-01 Impact factor: 37.312

Review 4. Physiological properties of the normal lens.

Authors: R T Mathias; J L Rae; G J Baldo
Journal: Physiol Rev Date: 1997-01 Impact factor: 37.312

5. Conditional mutations of beta-catenin and APC reveal roles for canonical Wnt signaling in lens differentiation.

Authors: Gemma Martinez; Mary Wijesinghe; Kirsty Turner; Helen E Abud; Makoto M Taketo; Tetsuo Noda; Michael L Robinson; Robb U de Iongh
Journal: Invest Ophthalmol Vis Sci Date: 2009-06-10 Impact factor: 4.799

Review 6. Exploring mechanisms of FGF signalling through the lens of structural biology.

Authors: Regina Goetz; Moosa Mohammadi
Journal: Nat Rev Mol Cell Biol Date: 2013-02-13 Impact factor: 94.444

7. Loss of function and impaired degradation of a cataract-associated mutant connexin50.

Authors: Viviana M Berthoud; Peter J Minogue; Jun Guo; Edward K Williamson; Xiaorong Xu; Lisa Ebihara; Eric C Beyer
Journal: Eur J Cell Biol Date: 2003-05 Impact factor: 4.492

8. Lens metabolic cooperation: a study of mouse lens transport and permeability visualized with freeze-substitution autoradiography and electron microscopy.

Authors: D A Goodenough; J S Dick; J E Lyons
Journal: J Cell Biol Date: 1980-08 Impact factor: 10.539

9. Targeted ablation of connexin50 in mice results in microphthalmia and zonular pulverulent cataracts.

Authors: T W White; D A Goodenough; D L Paul
Journal: J Cell Biol Date: 1998-11-02 Impact factor: 10.539

Review 10. A Network Map of FGF-1/FGFR Signaling System.

Authors: Rajesh Raju; Shyam Mohan Palapetta; Varot K Sandhya; Apeksha Sahu; Abbas Alipoor; Lavanya Balakrishnan; Jayshree Advani; Bijesh George; K Ramachandra Kini; N P Geetha; H S Prakash; T S Keshava Prasad; Yu-Jung Chang; Linyi Chen; Akhilesh Pandey; Harsha Gowda
Journal: J Signal Transduct Date: 2014-04-16

14 in total

Review 1. The lens growth process.

Authors: Steven Bassnett; Hrvoje Šikić
Journal: Prog Retin Eye Res Date: 2017-04-11 Impact factor: 21.198

2. The Phosphoinosotide 3-Kinase Catalytic Subunit p110α is Required for Normal Lens Growth.

Authors: Caterina Sellitto; Leping Li; Ehsan Vaghefi; Paul J Donaldson; Richard Z Lin; Thomas W White
Journal: Invest Ophthalmol Vis Sci Date: 2016-06-01 Impact factor: 4.799

Review 3. Focus on lens connexins.

Authors: Viviana M Berthoud; Anaclet Ngezahayo
Journal: BMC Cell Biol Date: 2017-01-17 Impact factor: 4.241

Differential regulation of Connexin50 and Connexin46 by PI3K signaling.

1. Expression of gap junctional proteins in Xenopus oocyte pairs.

2. A Calpha model for the transmembrane alpha helices of gap junction intercellular channels.

Review 3. Lens gap junctions in growth, differentiation, and homeostasis.

Review 4. Physiological properties of the normal lens.

5. Conditional mutations of beta-catenin and APC reveal roles for canonical Wnt signaling in lens differentiation.

Review 6. Exploring mechanisms of FGF signalling through the lens of structural biology.

7. Loss of function and impaired degradation of a cataract-associated mutant connexin50.

8. Lens metabolic cooperation: a study of mouse lens transport and permeability visualized with freeze-substitution autoradiography and electron microscopy.

9. Targeted ablation of connexin50 in mice results in microphthalmia and zonular pulverulent cataracts.

Review 10. A Network Map of FGF-1/FGFR Signaling System.

Review 1. The lens growth process.

2. The Phosphoinosotide 3-Kinase Catalytic Subunit p110α is Required for Normal Lens Growth.

Review 3. Focus on lens connexins.

Review 4. Connexin Mutants Compromise the Lens Circulation and Cause Cataracts through Biomineralization.

Review 5. PI3k and Stat3: Oncogenes that are Required for Gap Junctional, Intercellular Communication.

6. PI3K-Yap activity drives cortical gyrification and hydrocephalus in mice.

Review 7. Regulation of gap junction channels and hemichannels by phosphorylation and redox changes: a revision.

8. Connexin 50 Regulates Surface Ball-and-Socket Structures and Fiber Cell Organization.

9. Knock-in of Cx46 partially rescues fiber defects in lenses lacking Cx50.

10. Connexin 50-R205G Mutation Perturbs Lens Epithelial Cell Proliferation and Differentiation.